کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4515853 1322327 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular characterization of Glu-B3 locus in wheat cultivars and segregating populations
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Molecular characterization of Glu-B3 locus in wheat cultivars and segregating populations
چکیده انگلیسی


• The LMW-GS molecular marker method has been applied to segregating populations.
• The molecular marker system is a powerful tool for allelic determination of LMW-GS.
• The system can support variety identification and selection in breeding programs.

Bread wheat quality constitutes a key trait for the demands of the baking industry as well as the broad consumer preferences. The role of the low molecular weight glutenin subunits (LMW-GS) with regard to bread quality is so far not well understood owing to their genetic complexity and to the use of different nomenclatures and standards for the LMW-GS assignment by different research groups, which has made difficult the undertaking of association studies between genotypes and bread quality. The development of molecular markers to carry out genetic characterization and allele determination is demanding. Nowadays, the most promising LMW gene marker system is based on PCR and high resolution capillary electrophoresis for the simultaneous analysis of the complete multigene family. The molecular analysis of the bread wheat Glu-B3 locus in F2 and F4:6 populations expressed the expected one-locus Mendelian segregation pattern, thus validating the suitability of this marker system for the characterization of LMW-GS genes in segregating populations, allowing for the successful undertaking of studies related to bread-making quality. Moreover, the Glu-B3 allele characterization of standard cultivars with the molecular marker system has revealed its potential as a complementary tool for the allelic determination of this complex multigene family.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Cereal Science - Volume 60, Issue 2, September 2014, Pages 374–381
نویسندگان
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