کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4570377 1332025 2009 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The Interaction of Spinach Nitrite Reductase with Ferredoxin: A Site-Directed Mutation Study
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
The Interaction of Spinach Nitrite Reductase with Ferredoxin: A Site-Directed Mutation Study
چکیده انگلیسی

ABSTRACTA series of site-directed mutants of the ferredoxin-dependent spinach nitrite reductase has been characterized and several amino acids have been identified that appear to be involved in the interaction of the enzyme with ferredoxin. In a complementary study, binding constants to nitrite reductase and steady-state kinetic parameters of site-directed mutants of ferredoxin were determined in an attempt to identify ferredoxin amino acids involved in the interaction with nitrite reductase. The results have been interpreted in terms of an in-silico docking model for the 1:1 complex of ferredoxin with nitrite reductase.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 2, Issue 3, May 2009, Pages 407–415
نویسندگان
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