کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4570458 | 1332038 | 2013 | 12 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Nitric Oxide Mediates Cytokinin Functions in Cell Proliferation and Meristem Maintenance in Arabidopsis Nitric Oxide Mediates Cytokinin Functions in Cell Proliferation and Meristem Maintenance in Arabidopsis](/preview/png/4570458.png)
ABSTRACTCytokinin and nitric oxide (NO) have been characterized as signaling molecules to trigger cell division in tissue culture. Here, we show that the hypocotyl and root explants of Arabidopsis NO-deficient mutant nos1/noa1 exhibit severe defects in callus induction and shoot regeneration in response to cytokinin. Accordingly, depletion of NO caused by a NO scavenger leads to a severe inhibitory effect on callus induction. Moreover, cytokinin-induced NO production is impaired in nos1/noa1 in which cytokinin-triggered activation of cell cycle gene CYCD3;1 is inhibited, indicating that NO may act downstream of cytokinin in the control of cell proliferation through CYCD3;1. This hypothesis is further confirmed by the genetic evidence that constitutive expression of CYCD3;1 complements the defects of nos1/noa1 mutant in meristematic activity in shoot, root, and floral tissues as well as in cytokinin-induced callus initiation and shoot regeneration. Furthermore, we show that NO deficiency caused by loss of NOS1/NOA1 impairs cellular development such as the duration of the mitotic phase and timing of the transition to endocycles in nos1/noa1 mutant leaves, which can be reverted by constitutive expression of CYCD3;1. Taken together, these results demonstrate that NO mediates transcriptional activation of CYCD3;1 in regulating the mitotic cycles in response to cytokinins.
Journal: - Volume 6, Issue 4, July 2013, Pages 1214–1225