کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4752888 | 1416374 | 2017 | 42 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Heterologous expression in Pichia pastoris and characterization of a β-glucosidase from the xylophagous cockroach Panesthia angustipennis spadica displaying high specific activity for cellobiose
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کلمات کلیدی
CBBglycopeptidase Fp-nitrophenyl β-d-glucopyranosidepNPGGPFPNPCBHTLCCoomassie Brilliant Blue - Coomassie درخشان آبیp-Nitrophenol - p-nitrofenolβ-glucosidase - β-گلوکوزیدازAmino acids - اسید آمینه یا آمینو اسیدEndo H - اندو هendoglycosidase H - اندوگلیکوزیداز HEndoglucanase - اندوگلیکونازHeterologous expression - بیان هترولوژیکGlucose tolerance - تحمل گلوکزcellobiohydrolase - سلولی بیو هیدرولازPichia pastoris - پیکیا پاستوریسthin layer chromatography - کروماتوگرافی لایه نازک
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A β-glucosidase (BG), PaBG1b, from the xylophagous cockroach Panesthia angustipennis spadica was heterologously expressed in the methylotrophic yeast Pichia pastoris, purified, and biochemically characterized. Post-translational modification and N-terminal sequencing analysis demonstrated that the expression product was comprised of two polypeptides with different N-terminal sequences, presumably due to the presence of lysine-arginine (KR) sequence in the putative mature region. Substrate specificity analysis showed that PaBG1b hydrolyzed a broad range of substrates including cellohexaose, with the preference for aryl β-d-fucosyl linkage and laminaribiose. Although the glucose tolerance of PaBG1b was moderate (Ki = 200.3 ± 1.1 mM), PaBG1b demonstrated high specific activity and catalytic efficiency towards cellobiose with Vmax and kcat/Km values of 436.7 ± 6.3 U/mg and 109.8 mMâ1 sâ1, respectively. In addition, PaBG1b was not inhibited by cellobiose up to the highest concentration tested (100 mM). Collectively, our work demonstrates that PaBG1b is a potentially valuable BG for commercial bioethanol production from cellulose.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 97, February 2017, Pages 104-113
Journal: Enzyme and Microbial Technology - Volume 97, February 2017, Pages 104-113
نویسندگان
Yihai Li, Gaku Arakawa, Gaku Tokuda, Hirofumi Watanabe, Manabu Arioka,