کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4754618 1418069 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gardenia jasminoides extract-capped gold nanoparticles reverse hydrogen peroxide-induced premature senescence
ترجمه فارسی عنوان
نانوذرات طلا استخراج شده از گیاهان جاسمینوئیدس، تغییرات پیری زودرس ناشی از پراکسید هیدروژن
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


- Green synthesis of G. jasminoides-capped gold nanoparticles (GJ-GNPs) is presented.
- GJ-GNPs reduce lysosome content and ROS production in ARPE19 cells.
- GJ-GNPs inhibit SA-β-gal staining and activity in ARPE19 cells.
- GJ-GNPs induce expression of p53, p21, and SIRT3 in ARPE19 cells.
- GJ-GNPs induce HO-1 and NQO1 expression in ARPE19 cells.

This study reports a green approach for synthesis of gold nanoparticles using Gardenia jasminoides extract, and specifically, can potentially enhance anti senescence activity. Biological synthesis of gold nanoparticles is ecofriendly and effective for the development of environmentally sustainable nanoparticles compared with existing methods. Here, we developed a simple, fast, efficient, and ecofriendly approach to the synthesis of gold nanoparticles by means of a Gardenia jasminoides extract. These G. jasminoides extract-capped gold nanoparticles (GJ-GNPs) were characterized by UV-vis, high resolution transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), and Furrier transform infrared spectroscopy (FT-IR). The synthesized GJ-GNPs turned red and showed maximal absorbance at 540 nm. Thus, GJ-GNPs were synthesized successfully. We hypothesized that GJ-GNPs would protect ARPE19 cells from hydrogen peroxide-induced premature senescence. SA-β-gal activity was elevated in hydrogen peroxide-treated cells, however, this effect was attenuated by GJ-GNP treatment. Moreover, compared with the normal control, hydrogen peroxide treatment significantly increased lysosome content of the cells and production of reactive oxygen species (ROS). GJ-GNPs effectively attenuated the increase in lysosome content and ROS production in these senescent cells. According to cell cycle analysis, G2/M arrest was promoted by hydrogen peroxide treatment in ARPE19 cells, however, this change was reversed by GJ-GNPs. Western blot analysis showed that treatment with GJ-GNPs increased the expression of p53, p21, SIRT3, HO-1, and NQO1 in senescent cells. Our findings should advance the understanding of premature senescence and may lead to therapeutic use of GJ-GNPs in retina-related regenerative medicine.

Graphical Abstract

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 164, November 2016, Pages 204-211
نویسندگان
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