کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5040939 | 1473908 | 2017 | 11 صفحه PDF | دانلود رایگان |
- Tollip deficiency exacerbated neuronal cell death in mouse brain.
- Tollip deficiency caused accumulation of β amyloid and α-synuclein.
- Tollip deficiency affected autophagy as manifested in elevated levels of p62 and Parkin.
The excessive accumulation of specific cellular proteins or autophagic vacuoles (AVs) within neurons is a pathologic hallmark of neurodegenerative diseases. Constitutive autophagy in neurons prevents abnormal intracellular protein aggregation and is critical for maintaining cell survival. Since our previous study showed that Toll-interacting protein (Tollip)-deficient macrophages had constitutive disruption of endosome-lysosome fusion, we hypothesize that Tollip deficiency may also promote neuron death via blockage of autophagy completion. Indeed, we observed significantly higher levels of neuron death in the brain regions of cerebral cortex, hippocampus, and cerebellum from ApoEâ/â/Tollipâ/â mice as compared to ApoEâ/â mice fed with high fat diet (HFD). We further documented diminished density of neurons and increased ratios of TUNEL positive cells in the hippocampus of ApoEâ/â/Tollipâ/â mice. The ultrastructural electron microscopy analyses revealed neuron cell shrinkage as well as loss of intracellular structure in brain tissues from ApoEâ/â/Tollipâ/â mice. There was dramatic accumulation of autophagosomes in the cytoplasm, elevated accumulation of β-amyloid and α-synuclein, and increased levels of p62 and Parkin in the brain tissues from ApoEâ/â/Tollipâ/â mice as compared to ApoEâ/â mice. Our data suggest that Tollip may play a crucial role in sustaining neuron health by facilitating the completion of autophagy, and that Tollip-deficiency may accelerate neuron death related to neurological disease such as Alzheimer's disease.
Journal: Brain, Behavior, and Immunity - Volume 59, January 2017, Pages 200-210