کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5131433 | 1490893 | 2016 | 9 صفحه PDF | دانلود رایگان |
- An electrochemical aptasensor for the detection of Malathion has been developed.
- Chitosan-iron oxide NP deposited FTO sheets provides platform for aptamer immobilization.
- Aptasensor has efficiency to detect malathion upto 0.001Â ng/mL within 15Â min.
An electrochemical aptasensor based on chitosan-iron oxide nanocomposite (CHIT-IO) film deposited on fluorine tin Oxide (FTO) was developed for the detection of malathion. Iron oxide nanoparticles were prepared by co-precipitation method and characterized by Transmission electron microscopy and UV-Visible spectroscopy. The biotinylated DNA aptamer sequence specific to the malathion was immobilized onto the iron oxide doped-chitosan/FTO electrode by using streptavidin as linking molecule. Various characterization studies like Field Emission-Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR), and Electrochemical studies were performed to attest the successful fabrication of bioelectrodes. Experimental parameters like aptamer concentration, response time, stability of electrode and reusability studies were optimized. Aptamer immobilized chitosan-iron oxide nanocomposite (APT/SA/CHIT-IO/FTO) bioelectrodes exhibited LOD of about 0.001Â ng/mL within 15Â min and spike-in studies revealed about 80-92% recovery of malathion from the lettuce leaves and soil sample.
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Journal: Analytica Chimica Acta - Volume 939, 5 October 2016, Pages 108-116