کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5136757 | 1494014 | 2017 | 35 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Observation of the side chain O-methylation of glutamic acid or aspartic acid containing model peptides by electrospray ionization-mass spectrometry
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کلمات کلیدی
PTMiPrOHO-methylationCBBEtOHESI-MSCIDLC–MS/MS - LC-MS / MSMS/MS - MS / MSAspartic acid - آسپارتیک اسدEthanol - اتانولCollision-induced dissociation - اختلاف ناشی از برخوردAcetic acid - اسید استیکFormic acid - اسید فرمیکPyroglutamic acid - اسید پری گلوتامیکglutamic acid - اسید گلوتامیکposttranslational modification - اصلاح posttranslationalIsopropanol - ایزوپروپانول، ایزوپروپیل الکلElectrospray ionization-mass spectrometry - طیف سنجی جرم یونیزه الکترو اسپریTandem mass spectrometry - طیف سنجی جرمی پشت سر هم یا متوالیLiquid chromatography-tandem mass spectrometry - طیف سنجی جرمی کروماتوگرافی مایع دو طرفهMALDI - مالدیMethanol - متانولMeOH - مونmatrix-assisted laser desorption ionization - یونیزاسیون لیزر جذب ماتریس
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Observation of the side chain O-methylation of glutamic acid or aspartic acid containing model peptides by electrospray ionization-mass spectrometry Observation of the side chain O-methylation of glutamic acid or aspartic acid containing model peptides by electrospray ionization-mass spectrometry](/preview/png/5136757.png)
چکیده انگلیسی
O-methylation of the side chains of glutamic acid (E) and aspartic acid (D) residues is generally observed modification when an acidified methanol/water (MeOH/dH2O) mixture is used as a solvent system during sample preparation for proteomic research. This chemical modification may result misidentification with endogenous protein methylation; therefore, a special care should be taken during sample handling prior to mass spectrometric analysis. In the current study, we systematically examined the extent of E/D methylation and C-terminus carboxyl group of synthetic model peptides in terms of different incubation temperatures, storage times, and added acid types as well as its percentages. To monitor these effects, C-terminus amidated and free acid forms of synthetic model peptides comprised of E or D residue(s) have been analyzed by electrospray ionization-mass spectrometry (ESI-MS). Additionally, LC-MS/MS experiments were performed to confirm the formation of methylated peptide product. The results showed that the rate of methylation was increased as the temperature increases along with prolong incubation times. Moreover, the extent of methylation was remarkably high when formic acid (FA) used as a protonation agent instead of acetic acid (AA). In addition, it was found that the degree of methylation was significantly decreased by lowering acid percentages in ESI solution. More than one acidic residue containing model peptides have been also used to explore the extent of multiple methylation reaction. Lastly, the ethanol (EtOH) and isopropanol (iPrOH) have been substituted separately with MeOH in sample preparation step to investigate the extent of esterification reaction under the same experimental conditions. However, in the positive perspective of view, this method can be used as a simple, rapid and cheap method for methylation of acidic residues under normal laboratory conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1047, 15 March 2017, Pages 75-83
Journal: Journal of Chromatography B - Volume 1047, 15 March 2017, Pages 75-83
نویسندگان
A. Emin Atik, Melda Z. Guray, Talat Yalcin,