کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5137009 | 1494485 | 2017 | 8 صفحه PDF | دانلود رایگان |

- HPLC-DAD method was developed for the determination of isoflavones in soy products.
- ILUAE procedure was optimised by evaluating the influence of several parameters.
- ILUAE: 0.2Â g of sample soaked in 2Â mL of [C6MIM]Br and ultrasonicated for 40Â min.
- ILUAE method proposed shows potential as an environmentally-friendly technique.
- ILUAE-HPLC-DAD was used for the analysis of isoflavones in food samples.
An ionic liquid-based ultrasound-assisted extraction (ILUAE) was developed to extract four isoflavones, genistin, genistein, daidzin, and daidzein, from soy products. Various 1-alkyl-3-methylimidazolium compounds were investigated and 1-hexyl-3-methyl-imidazolium bromide ([C6MIM]Br) (1 M) was selected as the solvent. Several extraction variables, such as ionic liquid concentration, extraction time and solid/liquid ratio were also investigated. A method to determine the isoflavones by high-performance liquid chromatography (HPLC)-diode-array detection (DAD) was developed. Under the optimal extraction and chromatographic conditions, the isoflavones exhibited a good linear relationship, between 2.5-50 μg/g for daidzin and 0.5-50 μg/g for genistin, genistein and daidzein. The precision and accuracy of the proposed method wereâ<7% and between â8.5âandâ12%, respectively. The proposed method was successfully used to analyse soy food samples and the spiked recoveries ranged from 85.3 to 104%.
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Journal: Journal of Food Composition and Analysis - Volume 57, April 2017, Pages 94-101