A label-free sensitive method for membrane protein detection based on aptamer and AgNCs transfer

A strategy for membrane protein tyrosine kinase-7 in situ detection based on the protein triggered C-rich ssDNA sequence release and the silver nanoclusters transfer between the polymer template and C-rich sequence was developed. It shows a detection range from 30 pM to 2 nM with a detection limit of 12 pM. This strategy can realize quantitative detection simply and conveniently comparing with other methods which rely on complex instrumentation.215