Activity of cholesterol oxidase immobilized on Layered Double Hydroxide nanomaterials for biosensor application: Acacia salicina scavenging power of hypercholesterolemia therapy

• A highly sensitive cholesterol biosensor based on hybrid nanomaterials.
• A large linear range of the biosensor that covers all concentrations of cholesterol relevant to human health.
• A long term stability of the cholesterol biosensor.
• High inhibitory effect of phytosterol extracted from Acacia salicina vs enzymatic activity of cholesterol oxidase.

In the current study, we aimed to immobilize cholesterol oxidase (ChOx) on Layered Double Hydroxide (LDH) for cholesterol sensing. Obtained results have shown that immobilized ChOx keeps its activity even after several uses. However, some free radicals, such as hydrogen peroxide (H2O2), were generated through the enzymatic activity. In this work, we investigated the effects of hexane extract from Acacia salicina toward free radical scavenging activity. Hexane extract was an effective inhibitor of H2O2 generated by the enzymatic cholesterol/cholesterol oxidase catabolism (inhibition percentage ≈ 40%). This activity could be attributed to the presence of phytosterols in this extract. The present study demonstrates that extracts of A.salicina leaves are a potential source of antioxidant agents (most likely sterols compounds), their antioxidant activity might be used in chemoprevention trials.

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