کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5398825 | 1505893 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Interaction of biocompatible natural rosin-based surfactants with human serum albumin: A biophysical study
ترجمه فارسی عنوان
تعامل متقابل روتین واکنش های طبیعی با رزین با آلبومین سرم انسان: یک مطالعه بیوفیزیکی
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کلمات کلیدی
ITCHAShuman serum albumin - آلبومین سرم انسانیFörster resonance energy transfer - انتقال انرژی تابشی ForsterFRET - انتقال انرژی رزونانسی فورسترcircular dichroism - رنگ تابی دورانیHuman health - سلامتی انسانSurfactant binding - پیوند سطحیMolecular docking - پیوندهای بین مولکولیIsothermal titration calorimetry - کالری سنجی تیتاسیون ایزوترمال
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
چکیده انگلیسی
Biophysical insight into interaction of biocompatible rosin-based surfactants with human serum albumin (HSA) was studied at physiological conditions using various spectroscopic, calorimetric and molecular docking approaches. The binding constant (Kb), enthalpy (ÎH0), entropy (ÎS0) and Gibbs free energy change (ÎG0) were calculated by spectroscopic and calorimetric method. We have also calculated the probability of energy transfer by FRET analysis. The circular dichroism study showed that the cationic surfactant QRMAE significantly altered the secondary structure of HSA as compared to the nonionic rosin surfactants. The thermodynamic study was performed by ITC to determine binding constant as well as change in enthalpy of HSA in presence of rosin surfactants. It clearly showed that hydrogen binding and hydrophobic interaction play an important role in the binding of HSA to rosin surfactants. We have also performed molecular docking studies to locate the binding site on HSA and to visualize the mode of interaction. The present study provides a significant insight into HSA-rosin surfactants interaction, which also improves our understanding of the possible effect of rosin surfactants on human health.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Luminescence - Volume 167, November 2015, Pages 399-407
Journal: Journal of Luminescence - Volume 167, November 2015, Pages 399-407
نویسندگان
Mohd Ishtikhar, Mohd. Sajid Ali, Ayman M. Atta, H.A. Al-Lohedan, Lokesh Nigam, Naidu Subbarao, Rizwan Hasan Khan,