کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5420989 | 1507517 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Solid-state NMR structural studies of the fibril form of a mutant mouse prion peptide PrP89-143(P101L)
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کلمات کلیدی
PrPDouble-quantum NMRβ-helixMultiple-quantum NMRCJDAβCPMGBSECarr–Purcell–Meiboom–Gill - Carr Purcell-Meiboom-GillSolid-state NMR - NMR حالت جامدBovine spongiform encephalopathy - جنون گاویDouble quantum - دو کوانتومیElectron microscopy - میکروسکوپ الکترونیAlignment - هم ترازیPrion protein - پروتئین پریونPrion - پریونamyloid beta peptide - پپتید بتا آمیلوئید
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The peptide fragment 89-143 of the prion protein (carrying a P101L mutation) is biologically active in transgenic mice when in a fibrillar form. Injection of these fibrils into transgenic mice (expressing full length PrP with the P101L mutation) induces a neurodegenerative prion disease (Kaneko et al., J. Mol. Biol. 295 (2000) 997). Here we present solid-state NMR studies of PrP89-143(P101L) fibrils, probing the conformation of residues in the hydrophobic segment 112-124 with chemical shifts. The conformations of glycine residues were analyzed using doubly 13C=O labeled peptides by two-dimensional (2D) double-quantum correlation, and double-quantum filtered dephasing distance measurements. MQ-NMR experiments were carried out to probe the relative alignment of the individual peptides fibrils. These NMR studies indicate that the 112-124 segment adopts an extended β-sheet conformation, though not in a parallel, in register alignment. There is evidence for conformational variability at Gly 113. DQ correlation experiments provide useful information in regions with conformational heterogeneity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Solid State Nuclear Magnetic Resonance - Volume 29, Issues 1â3, February 2006, Pages 183-190
Journal: Solid State Nuclear Magnetic Resonance - Volume 29, Issues 1â3, February 2006, Pages 183-190
نویسندگان
Kwang Hun Lim, Tuan N. Nguyen, Steven M. Damo, Tanya Mazur, Haydn L. Ball, Stanley B. Prusiner, Alexander Pines, David E. Wemmer,