کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5510494 | 1539130 | 2017 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of mitochondrial glycerol-3-phosphate acyltransferase in notothenioid fishes
ترجمه فارسی عنوان
تشخیص گلیسرول 3-فسفات آیتیل ترانسفراز میتوکندری در ماهیان نوتنیوئید
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کلمات کلیدی
Elongation factor-1 alphaLPANRF-1PGC-1TBP18SEF-1αGPATG3PLPAATHKGdN/dS - DN / DS18S ribosomal RNA - RNA ریبوزوم 18Sphosphatidic acid - اسید فسفاتیدیکlysophosphatidic acid - اسید لیسفسفیدیدtriacylglycerol - تری آسیل گلیسرول TAG یا triacylglycerols - تری گلیسرید یا تری آسیل گلیسرولdiacylglycerol - دیسیل گلیسیرینDAG - روزCold adaptation - سازگاری سردphosphatidylinositol - فسفاتیدیل اینوزیتولphosphatidylcholine - فسفاتیدیل کولینphosphatidylethanolamine - فسفاتیدیلتانولامینPhosphatidylserine - فسفاتیدیلسرینPhospholipids - فسفولیپیدlysophosphatidic acid acyltransferase - لیسفسفید فسفیدید اسید آتیل ترانسفرازFishes - ماهی هاMitochondria - میتوکندریاTATA-binding protein - پروتئین متصل به TATAHousekeeping gene - ژن خانگیglycerol 3-phosphate - گلیسرول 3-فسفات
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Hearts of Antarctic icefishes (suborder Notothenioidei, family Channichthyidae) have higher densities of mitochondria, and mitochondria have higher densities of phospholipids, compared to red-blooded notothenioids. Glycerol-3-phosphate acyltransferase (GPAT) catalyzes the rate-limiting step in glycerolipid biosynthesis. There are four isoforms of GPAT in vertebrates; GPAT1 and GPAT2 are localized to the outer mitochondrial membrane, whereas GPAT3 and GPAT4 are localized to the endoplasmic reticulum membrane. We hypothesized that transcript levels of GPAT1 and/or GPAT2 would mirror densities of mitochondrial phospholipids and be higher in the icefish Chaenocephalus aceratus compared to the red-blooded species Notothenia coriiceps. Transcript levels of GPAT1 were quantified in heart ventricles and liver using qRT-PCR. Additionally, GPAT1 cDNA was sequenced in the Antarctic notothenioids, C. aceratus and N. coriiceps, and in the sub-Antarctic notothenioid, Eleginops maclovinus, to identify amino acid substitutions that may maintain GPAT1 function at cold temperature. Transcript levels of GPAT1 were higher in liver compared to heart ventricles but were not significantly different between the two species. In contrast, transcripts of GPAT2 were only detected in ventricle where they were 6.6-fold higher in C. aceratus compared to N. coriiceps. These data suggest GPAT1 may be more important for synthesizing triacylglycerol, whereas GPAT2 may regulate synthesis of phospholipids. GPAT1 amino acid sequences are highly conserved among the three notothenioids with 97.9-98.7% identity. Four amino acid substitutions within the cytosolic region of Antarctic notothenioid GPAT1 may maintain conformational changes necessary for binding and catalysis at cold temperature.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 204, February 2017, Pages 9-26
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 204, February 2017, Pages 9-26
نویسندگان
Kelly A. Keenan, Theresa J. Grove, Corey A. Oldham, Kristin M. O'Brien,