کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5511350 | 1539853 | 2017 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Genes directly regulated by NF-κB in human hepatocellular carcinoma HepG2
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کلمات کلیدی
RNA-seqDTGsChIP-SeqRPKMFold enrichmentTESDBPHepG2TSSTNFαtranscriptional start sitesNF-κBFBSLPSDMEMHCC - HCCPMA - LDC هاSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNASTAT - آمارCellular components - اجزای سلولیchromatin immunoprecipitation - ایمن سازی کروماتینMolecular function - تابع مولکولیtumor necrosis factor α - تومور نکروز عامل αKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوfetal calf serum - سرم گوساله جنینDEG - شماTranscription factor - عامل رونویسیnuclear factor κB - فاکتور هسته ای κBBiological processes - فرآیندهای بیولوژیکیphorbol myristate acetate - فروبل مریستات استاتlipopolysaccharide - لیپوپلی ساکاریدSignal transducer and activator of transcription - مبدل سیگنال و فعال کننده رونویسیDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده DulbeccoGene ontology - هستیشناسی ژنیHBV - هپاتیت بHepatitis C virus - هپاتیت سیHCV - هپاتیت سیhepatitis B virus - ویروس هپاتیت بیCHiP - چیپDifferentially expressed gene - ژن بیان شده متفاوت استTarget gene - ژن هدفHepatocellular carcinoma - کارسینوم هپاتوسلولار(کارسینوم سلولهای استخوانی)
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
It has been well-known that over activation of NF-κB has close relationship with hepatitis and hepatocellular carcinoma (HCC). However, the complete and exact underlying molecular pathways and mechanisms still remain not fully understood. By manipulating NF-κB activity with its recognized activator TNFα and using ChIP-seq and RNA-seq techniques, this study identified 699 NF-κB direct target genes (DTGs) in a widely used HCC cell line, HepG2, including 399 activated and 300 repressed genes. In these NF-κB DTGs, 216 genes (126 activated and 90 repressed genes) are among the current HCC gene signature. In comparison with NF-κB target genes identified in LPS-induced THP-1 and TNFα-induced HeLa cells, only limited numbers (24-46) of genes were shared by the two cell lines, indicating the HCC specificity of identified genes. Functional annotation revealed that NF-κB DTGs in HepG2 cell are mainly related with many typical NF-κB-related biological processes including immune system process, response to stress, response to stimulus, defense response, and cell death, and signaling pathways of MAPK, TNF, TGF-beta, Chemokine, NF-kappa B, and Toll-like receptor. Some NF-κB DTGs are also involved in Hepatitis C and B pathways. It was found that 82 NF-κB DTGs code secretory proteins, which include CCL2 and DKK1 that have already been used as HCC markers. Finally, the NF-κB DTGs were further confirmed by detecting the NF-κB binding and expression of 14 genes with ChIP-PCR and RT-PCR. This study thus provides a useful NF-κB DTG list for future studies of NF-κB-related molecular mechanisms and theranostic biomarkers of HCC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 89, August 2017, Pages 157-170
Journal: The International Journal of Biochemistry & Cell Biology - Volume 89, August 2017, Pages 157-170
نویسندگان
Wei Dai, Jian Wu, Shuyan Zhang, Bingjie Shi, Xinhui Xu, Danyang Wang, Jinke Wang,