کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5514252 1541596 2017 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nitrite increases glucose-stimulated insulin secretion and islet insulin content in obese type 2 diabetic male rats
ترجمه فارسی عنوان
نیتیتین باعث افزایش ترشح انسولین تحریک شده توسط گلوکز و محتوای انسولین جزیره در موشهای صحرایی نابارور نوع 2 چاق
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


- Nitrite increases glucose-stimulated insulin secretion in control and diabetic rats.
- Nitrite increases islet insulin content in both control and diabetic rats.
- Nitrite improves glucose tolerance and insulin sensitivity in diabetic rats.
- Nitrite improves dyslipidemia in obese type 2 diabetic rats.
- Nitrite decreases inflammation in obese type 2 diabetic rats.

PurposeReduced bioavailability of nitric oxide (NO) is associated with pathogenesis of type 2 diabetes. Nitrite can act as a substrate for generation of systemic NO. The aim of this study was to examine the effects of nitrite administration on glucose-stimulated insulin secretion (GSIS) and islet insulin content in obese type 2 diabetic rats.MethodsMale rats were divided into 4 groups: Control, control + nitrite, diabetes, and diabetes + nitrite. Sodium nitrite (50 mg/L in drinking water) was administered for 8 weeks. Diabetes was induced using high-fat diet and low-dose of streptozotocine. Serum levels of fasting glucose, insulin, and lipid profile were measured and the insulin resistance/sensitivity indices were calculated every 2 weeks. Glycated hemoglobin (HbA1C) was measured every month. At the end of the study, tissue levels of glucose transporter 4 (GLUT4) protein and serum interleukin-1 beta (IL-1β) were measured as well as glucose and insulin tolerance test were done. GSIS from isolated pancreatic islets and islet insulin content were also determined.ResultsNitrite administration significantly increased insulin secretion in both control and diabetic rats in presence of 16.7 mM glucose. Nitrite also significantly increased islet insulin content by 27% and 39% in both control and diabetic rats, respectively. Nitrite decreased elevated serum IL-1β in diabetic rats (4.0 ± 0.2 vs. 2.9 ± 0.2 pg/mL, P = 0.001). In diabetic rats, nitrite also significantly increased tissue levels of GLUT4 by 22% and 26% in soleus muscle and epididymal adipose tissue, respectively. In addition, nitrite significantly improved glucose and insulin tolerance, insulin sensitivity, lipid profile, and decreased fasting glucose and insulin, but had no effect on HbA1C.ConclusionsLong-term nitrite administration increased both insulin secretion and insulin content in obese type 2 diabetic rats. In addition, nitrite therapy had favorable effects on glucose tolerance, insulin resistance, inflammation, and dyslipidemia in type 2 diabetic rats.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nitric Oxide - Volume 64, 1 April 2017, Pages 39-51
نویسندگان
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