کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5550941 | 1557304 | 2016 | 12 صفحه PDF | دانلود رایگان |

Here we examine the mechanisms by which nanoparticles enable the oral absorption of water-insoluble, P-glycoprotein efflux pump (P-gp) substrates, without recourse to P-gp inhibitors. Both 200 nm paclitaxel N-(2-phenoxyacetyl)-6-O-glycolchitosan (GCPh) nanoparticles (GCPh-PTX) and a simulated Taxol formulation, facilitate drug dissolution in biorelevant media, unlike paclitaxel nanocrystals. Verapamil (40 mg kgâ1) increased the oral absorption from low dose Taxol (6 or 10 mg kgâ1) by 100%, whereas the oral absorption from high dose Taxol (20 mg kgâ1) or low dose GCPh-PTX (6 or 10 mg kgâ1) was largely unchanged by verapamil. There was virtually no absorption from control paclitaxel nanocrystals (20 mg kgâ1). Imaging of ex-vivo rat ileum samples showed that fluorescently labelled GCPh nanoparticles are mucoadhesive and are taken up by ileum epithelial cells. GCPh nanoparticles were also found to open Caco-2 cell tight junctions. In conclusion, mucoadhesive, drug solubilising GCPh nanoparticles enable the oral absorption of paclitaxel via the saturation of the P-gp pump (by high local drug concentrations) and by particle uptake and tight junction opening mechanisms.
Rat ileum showing orally dosed particles (red) in the villi and basement membrane (yellow arrows) and adhering to the mucus (white arrows).133
Journal: International Journal of Pharmaceutics - Volume 514, Issue 1, 30 November 2016, Pages 121-132