کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5552673 | 1557949 | 2017 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Antiviral activity of a synthesized shikonin ester against influenza A (H1N1) virus and insights into its mechanism
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
FBSCPEDMEMCC50MDCKDMSO - DMSOMOI - MECytopathic effect - اثر سیتوپاتیکEsters - استرShikonin - تماشایDimethyl sulfoxide - دیمتیل سولفواکسیدfetal bovine serum - سرم جنین گاوMadin-Darby canine kidney cells - سلول های کلیوی کانین Madin-DarbyAntiviral agents - عوامل ضد ویروسیDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده Dulbecconeuraminidase - نورآمینیدازNucleoprotein - هسته پروتئینhemagglutinin - هماگلوتینینmultiplicity of infection - چندین عفونتDrug discovery - کشف مواد مخدر
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
تومور شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This study aimed to examine the antiviral effects of shikonin ester ((R)-1-(5, 8-dihydroxy-1,4-dioxo-1,4-dihydronaphthalen-2-yl)-4-methylpent-3-en-1-yl3-(1H- indol-3-yl) propanoate (PMM-034) against influenza A (H1N1) virus. We investigated PMM-034 anti-H1N1 activity and its effect on caspase 3 gene expression during cellular apoptosis after influenza virus infection in vitro. Neuraminidase (NA) inhibition was assessed in comparison with oseltamivir in the influenza virus standard strains A/PR/8/34 to understand the viral mechanism. MDCK and A549 cells were used to investigate influenza viral infection and the structure-activity relationship between PMM-034 and NA was evaluated by pharmacophore-based docking modeling. The production of viral protein was tested by western blot. A/PR/8/34 induced cell inhibition but this was reduced by PMM-034 to 16 μg/mL and this showed a selective index of 10 mM. PMM-034 inhibited NA in a dose dependent manner, similar to oseltamivir inhibition. A sharp decrease in viral nucleocapsid protein mRNA was observed in infected cells after treatment with PMM-034. Apoptosis of infected A459 cells was inhibited by PMM-034 with decreased caspase 3 levels. ARG 118, ARG 152, ARG 371 and GLU 227 in the binding pocket of NA bound to PMM-034 in the docking model. Taken together, these results suggest PMM-034 shikonin ester blocked H1N1 infection and might be a potential anti-H1N1 drug.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomedicine & Pharmacotherapy - Volume 93, September 2017, Pages 636-645
Journal: Biomedicine & Pharmacotherapy - Volume 93, September 2017, Pages 636-645
نویسندگان
Yahan Zhang, Hongwei Han, Hanyue Qiu, Hongyan Lin, Lugang Yu, Wanzhan Zhu, Jinliang Qi, Rongwu Yang, Yanjun Pang, Xiaoming Wang, Guihua Lu, Yonghua Yang,