کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5588865 | 1569141 | 2017 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cloning of invertebrate gonadotropin-releasing hormone receptor (GnRHR)-like gene in Yesso scallop, Patinopecten yessoensis
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کلمات کلیدی
ISHGnRHRFollicle stimulation hormoneAdipokinetic hormone receptorTR-FIAGPCRCpGAKHRACPRIn situ hybridization - Hybridization در محلVpr - VPROxtr - اکسترrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدCNS - دستگاه عصبی مرکزیBivalves - دوکفهایها central nervous system - سیستم عصبی مرکزیtransmembrane - فرابنفشRace - مسابقهMollusk - مولوسکIsoelectric point - نقطه ایزوالکتریکNeuropeptide - نوروپپتیدluteinizing hormone - هورمون جسم زردFSH - هورمون محرکه فولیکولی Molecular weight - وزن مولکولیoxytocin receptor - گیرنده اکسی توسینgonadotropin-releasing hormone receptor - گیرنده هورمون آزاد کننده گنادوتروپینVasopressin receptor - گیرنده واپسروزG protein-coupled receptor - گیرندههای جفتشونده با پروتئین جی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Although the invGnRH peptide was characterized from the nerve ganglia in bivalves, its signaling mechanism is nevertheless unclear. The objective of this paper was to identify the invGnRHR cDNA from Yesso scallop as a first step for understanding of invGnRH in bivalve neuroendocrine system. We performed PCR cloning mediated with transcriptome survey and expression analysis with various tissues of the scallop. Our results showed that we identified an invGnRHR-like cDNAs from not only Yesso scallop Patinopecten yessoensis but also Pacific oyster Crassostrea gigas. In addition, we subsequently identified an adipokinetic hormone receptor (AKHR)-like and AKH-like cDNA pair from the scallop. Comparison of the tissue distributions of both receptor mRNAs suggested functional divergence of two homologous neuropeptides. In brief, py-GnRHR-like mRNA showed broad distribution in various tissues including the nerve ganglia, while py-AKHR-like mRNA was expressed in the nerve ganglia and restricted to some limited peripheral tissues. The findings suggested that both py-GnRH and py-AKH signals were utilized via their own receptors. qPCR assays revealed their receptor mRNA expression in the gonads during a maturation, showing that py-GnRHR-like mRNA in the pre-mature gonads was higher than other mature stages.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Agri Gene - Volume 3, March 2017, Pages 46-56
Journal: Agri Gene - Volume 3, March 2017, Pages 46-56
نویسندگان
Kazue Nagasawa, Kouta Muroi, Tongchai Thitiphuree, Yuki Minegishi, Naoki Itoh, Makoto Osada,