کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589772 | 1569817 | 2017 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Genome wide identification of microRNAs involved in fatty acid and lipid metabolism of Brassica napus by small RNA and degradome sequencing
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کلمات کلیدی
SPLLong-chain acyl-CoA synthetaseAcyl-CoA synthetasesFATPBrassica napusACSLacyl-CoA oxidaseARFqRT-PCRDAFACOACSPDCRCAmicro RNA - RNA میکروsmall RNA - RNA کوچکFatty acid - اسید چربanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of variancetriacylglycerol - تری آسیل گلیسرول TAG یا triacylglycerols - تری گلیسرید یا تری آسیل گلیسرولHigh-throughput sequencing - توالی انتخابی بالاDegradome - خرابکاریKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو day after flowering - روز بعد از گلTranscript - رونوشتquantitative real time PCR - زمان واقعی PCR کمیSRNA - سرناAuxin response factor - فاکتور پاسخ اکسینMiRNA - میکروRNA، ریزآرانای، miRNAGene ontology - هستیشناسی ژنیFatty acid transport protein - پروتئین حمل و نقل اسید چربpyruvate dehydrogenase complex - پیرووات دهیدروژناز پیچیده
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Rapeseed (Brassica napus) is an important cash crop considered as the third largest oil crop worldwide. Rapeseed oil contains various saturation or unsaturation fatty acids, these fatty acids, whose could incorporation with TAG form into lipids stored in seeds play various roles in the metabolic activity. The different fatty acids in B. napus seeds determine oil quality, define if the oil is edible or must be used as industrial material. miRNAs are kind of non-coding sRNAs that could regulate gene expressions through post-transcriptional modification to their target transcripts playing important roles in plant metabolic activities. We employed high-throughput sequencing to identify the miRNAs and their target transcripts involved in fatty acids and lipids metabolism in different development of B. napus seeds. As a result, we identified 826 miRNA sequences, including 523 conserved and 303 newly miRNAs. From the degradome sequencing, we found 589 mRNA could be targeted by 236 miRNAs, it includes 49 novel miRNAs and 187 conserved miRNAs. The miRNA-target couple suggests that bna-5p-163957_18, bna-5p-396192_7, miR9563a-p3, miR9563b-p5, miR838-p3, miR156e-p3, miR159c and miR1134 could target PDP, LACS9, MFPA, ADSL1, ACO32, C0401, GDL73, PlCD6, OLEO3 and WSD1. These target transcripts are involving in acetyl-CoA generate and carbon chain desaturase, regulating the levels of very long chain fatty acids, β-oxidation and lipids transport and metabolism process. At the same, we employed the q-PCR to valid the expression of miRNAs and their target transcripts that involve in fatty acid and lipid metabolism, the result suggested that the miRNA and their transcript expression are negative correlation, which in accord with the expression of miRNA and its target transcript. The study findings suggest that the identified miRNA may play important role in the fatty acids and lipids metabolism in seeds of B. napus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 619, 1 July 2017, Pages 61-70
Journal: Gene - Volume 619, 1 July 2017, Pages 61-70
نویسندگان
Zhiwei Wang, Yan Qiao, Jingjing Zhang, Wenhui Shi, Jinwen Zhang,