کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5590072 | 1570095 | 2017 | 40 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparative analysis of MBD-seq and MeDIP-seq and estimation of gene expression changes in a rodent model of schizophrenia
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کلمات کلیدی
RNA-seqDMRMAMCpGCGIMeDIP-SeqSchizophrenia - اسکیزوفرنی یا شیزوفرنیbisulfite sequencing - توالی بیسولفیتCpG island - جزیره CpGCytosine-phosphate-Guanine - سیتوزین-فسفات-گوانینmethylazoxymethanol acetate - متیلازوکمتانول استاتDNA methylation - متیلاسیون DNADifferentially methylated region - منطقه متخلخل متفاوتی داردGene ontology - هستیشناسی ژنی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
We conducted a comparative study of multiplexed affinity enrichment sequence methodologies (MBD-seq and MeDIP-seq) in a rodent model of schizophrenia, induced by in utero methylazoxymethanol acetate (MAM) exposure. We also examined related gene expression changes using a pooled sample approach. MBD-seq and MeDIP-seq identified 769 and 1771 differentially methylated regions (DMRs) between F2 offspring of MAM-exposed rats and saline control rats, respectively. The assays showed good concordance, with ~Â 56% of MBD-seq-detected DMRs being identified by or proximal to MeDIP-seq DMRs. There was no significant overlap between DMRs and differentially expressed genes, suggesting that DNA methylation regulatory effects may act upon more distal genes, or are too subtle to detect using our approach. Methylation and gene expression gene ontology enrichment analyses identified biological processes important to schizophrenia pathophysiology, including neuron differentiation, prepulse inhibition, amphetamine response, and glutamatergic synaptic transmission regulation, reinforcing the utility of the MAM rodent model for schizophrenia research.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics - Volume 109, Issues 3â4, July 2017, Pages 204-213
Journal: Genomics - Volume 109, Issues 3â4, July 2017, Pages 204-213
نویسندگان
Jennifer L. Neary, Stephanie M. Perez, Kara Peterson, Daniel J. Lodge, Melanie A. Carless,