کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5649459 | 1407125 | 2016 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Activation of TGF-β1 by AQP3-Mediated H2O2 Transport into Fibroblasts of a Bleomycin-Induced Mouse Model of Scleroderma
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کلمات کلیدی
DPIPBSTGF-β1COL3Aquaporin 3AQP3BMFBLMshRNADCFH-DAPMF2′,7′-dichlorofluorescein diacetate - 2 '، 7'-dichlorofluorescein diacetateshort hairpin RNA - RNA موی سر کوتاهROS - ROSHydrogen peroxide - آب اکسیژنهBleomycin - بلئومایسینTransforming growth factor-β1 - تبدیل فاکتور رشد β1diphenyleneiodonium chloride - دی فینیلنیدونیم کلریدPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریH2O2 - هیدروژن پراکسیدCol1 - کل 1Collagen type I - کلاژن نوع Icollagen type III - کلاژن نوع IIIReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
امراض پوستی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Hydrogen peroxide (H2O2), the most important reactive oxygen species, mediates intracellular signal transmission and is transported into cells by aquaporin 3 (AQP3). However, it remains unclear whether AQP3 is involved in the pathogenesis of scleroderma. In this study, we examined the role of AQP3 in a bleomycin-induced mouse model of scleroderma. We observed that H2O2 and AQP3 levels in mouse skin increased with the bleomycin injection period relative to phosphate-buffered saline-injected control mice. AQP3 mRNA and protein levels were higher in bleomycin mice fibroblasts than in phosphate-buffered saline mice fibroblasts, and AQP3 immunofluorescence signals in the cytoplasm and cell membrane of bleomycin mice fibroblasts were much stronger than those in phosphate-buffered saline mice fibroblasts. Bleomycin-induced increases in H2O2, transforming growth factor-β1, collagen type I, and collagen type III levels in bleomycin mice fibroblasts were blocked by silencing AQP3. In addition, silencing AQP3 decreased H2O2 levels, transforming growth factor-β1 expression, and fibrosis in the scleroderma mouse model. These results demonstrate that AQP3-mediated transport of H2O2 into bleomycin mice fibroblasts activated transforming growth factor-β1, and silencing AQP3 is a potential approach for treating scleroderma.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Investigative Dermatology - Volume 136, Issue 12, December 2016, Pages 2372-2379
Journal: Journal of Investigative Dermatology - Volume 136, Issue 12, December 2016, Pages 2372-2379
نویسندگان
Jingying Luo, Xin Liu, Jie Liu, Miao Jiang, Miao Luo, Jingjun Zhao,