Bio-effect-monitoring of long-term thermal wastes on the oyster, Crassostrea gigas, using heat shock proteins

We bio-monitored the stress of oyster, Crassostrea gigas, for possible long term effects of thermal waste from a power plant. The expression level of its heat shock proteins (HSPs) was measured by real time-reverse transcript PCR along with their density and growth in the field. Oyster size varied in a distance dependent pattern. Physics modeling for evaluation of spreading of the thermal effluent revealed that station A is affected by the thermal effluents abundance, and the size of C. gigas showed a negative relationship with distance to the power plant. The abundance and size of C. gigas were smallest at station A, which was closest to the thermal effluent outlet. The kinetics of changes in the hsp70 and hsp90 mRNA levels in the mantle of C. gigas were also investigated. Regardless of the higher expression level of hsp70 mRNA than hsp90, both hsp70 and hsp90 mRNA levels were significantly higher at station A. The expression levels decreased inversely with distance from the thermal effluent outlet, with expression of hsp70 mRNA at station A being approximately 7-fold higher than at station B and 15-fold higher than at station C. Similarly, expression of hsp90 mRNA at station A was approximately 14-fold higher than at station B and 22-fold higher than at station C. The present findings provide new insights on biological correlation among the growth of individuals and population size and the molecular index in C. gigas following thermal effects.