Dual-dummy-template molecularly imprinted polymer combining ultra performance liquid chromatography for determination of fluoroquinolones and sulfonamides in pork and chicken muscle

In this study, a dual-dummy-template molecularly imprinted polymer capable of simultaneous recognizing 8 fluoroquinolones and 8 sulfonamides was synthesized. Its recognition performance was investigated by comparing the 3D conformations of four dummy templates and the two classes of drugs based on computational simulation. Then a solid phase extraction column was prepared and optimized that was combined with ultra performance liquid chromatography for determination of the 16 drugs in pork and chicken. The column could be reused for at least eighty times, and it showed high absorbency capacities (34.9-74.2 μg) and high recoveries (92%-99%) to these drugs. The limits of detection of this method for the two classes of drugs in meat were in the range of 1.0-3.4 ng/g, and the recoveries from the standards fortified blank samples were in the range of 86.1%-109.4%. Therefore, this method could be used as a specific, sensitive and accurate method for determination of fluoroquinolones and sulfonamides in meat.