کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5767157 | 1628382 | 2017 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An immunoassay to assess lamb and kid rennets authenticity
ترجمه فارسی عنوان
یک آزمایش ایمنی برای ارزیابی صحت تخم مرغ و جوجه های گوشتی
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کلمات کلیدی
تقلب فرانسوی بچه رژیم گوشت گوسفند، پپسین، ایمونوآسیا،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
چکیده انگلیسی
The potential adulteration of kid or lamb rennet with calf rennet is of interest for some Protected Designation of Origin cheeses producers and those looking for a specific cheese typicality. The approach proposed here for the authentication of kid or lamb rennet is based on the immuno-detection of bovine pepsin possibly present in calf rennet in varying quantities. The developed immunoassay (indirect ELISA) used a monoclonal antibody (mAb) raised against bovine pepsin. This mAb was found to be specific as it didn't cross-react with the pepsin of animal species other than bovine (kid, lamb, pig) and with other milk-clotting enzymes (chymosin and microbial enzymes). Adulteration tests were conducted with kid and lamb rennets spiked with a wide range of calf rennet (from 0 to 100% v/v). The presence of bovine pepsin was detected at low levels down to 6Â mg/L in kid and lamb rennets. Good linear relationships were obtained between added bovine pepsin concentration and the absorbance values over the range 1.25-120Â mg/L. Results showed that indirect ELISA proved to be an interesting tool for testing rennets authenticity targeting bovine pepsin as an indicator of the bovine adulteration of kid and lamb rennets.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 82, December 2017, Pages 251-255
Journal: Food Control - Volume 82, December 2017, Pages 251-255
نویسندگان
Odile Rolet-Répécaud, Céline Arnould, Stéphane Gavoye, Eric Beuvier, Christine Achilleos,