کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5801591 1555402 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of chicken egg yolk immunoglobulins (IgYs) specific for the most prevalent capsular serotypes of mastitis-causing Staphylococcus aureus
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Characterization of chicken egg yolk immunoglobulins (IgYs) specific for the most prevalent capsular serotypes of mastitis-causing Staphylococcus aureus
چکیده انگلیسی

The objective of this in vitro study was to evaluate the potential of egg yolk immunoglobulins (IgYs) for treating mastitis caused by Staphylococcus aureus. Specific IgY against type 5 (IgY-T5), type 8 (IgY-T8) and type 336 (IgY-T336) S. aureus strains were obtained by immunizing hens with whole cell vaccines and the IgY produced were then purified to around 80% purity using a water dilution method coupled with salting out and ultra-filtration. Enzyme-linked immunosorbent assay indicated that the IgY specifically targeted the three homologous strains. A growth inhibition assay was performed in Columbia broth (non-encapsulated form) and phosphate-buffered saline (encapsulated form) for an 8 h incubation. The results showed that IgY-T336 significantly inhibited (but only 1.5 log units; P < 0.01) the growth of all three strains at 15 mg/ml in the Columbia broth. In contrast, the same concentrations of IgY-T5 and IgY-T8 did not show obvious bacteriostatic activity against the two homologous strains. In phosphate buffered saline, no inhibition of the two encapsulated strains was observed with IgY-T5, IgY-T8 and IgY-T336. However, IgY-T336 reduced live bacteria by 1.0 log unit against strain 336 compared with the control. An internalization test indicated that all of the specific IgY (at 5 mg/ml) significantly (about 3.0 log units of the control; P < 0.01) blocked the internalization of their homologous strains by bovine mammary epithelial cells (MAC-T cells) within 6 h. These results suggested that research on the application of IgY as a treatment for mastitis caused by S. aureus should be focused on the internalization inhibition activity rather than on the growth inhibition activity of the IgY.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 149, Issues 3–4, 5 May 2011, Pages 415-421
نویسندگان
, , , , , , ,