Expression profile of bio-defense genes in Penaeus monodon gills in response to formalin inactivated white spot syndrome virus vaccine

- WSSV vaccine with a viral titer of 1 × 109 DNA copies ml−1 prepared.
- P. monodon vaccinated with 1.75 × 106 DNA copies ml−1 of IVP day−1 for 7 days.
- Challenged with WSSV on 1st and 5th day post vaccination.
- 100% and 50% survival observed respectively on 1st and 5th day post vaccination challenge.
- Up-regulation of proPO, α2M, PmRACK, Rab7, crustin and caspase genes observed.

White spot syndrome virus (WSSV) is the most devastating pathogen of penaeid shrimp. While developing technology to vaccinate shrimp against WSSV, it is imperative to look into the immune response of the animal at molecular level. However, very little information has been generated in this direction. The present study is an attempt to understand the expression of bio-defense genes in gill tissues of Penaeus monodon in response to formalin inactivated WSSV. A WSSV vaccine with a viral titer of 1 × 109 DNA copies was prepared and orally administered to P. monodon at a rate of 1.75 × 106 DNA copies of inactivated virus preparation (IVP) day−1 for 7 days. The animals were challenged with WSSV on 1st and 5th day post vaccination, and temporal expression of bio-defense genes in gill tissues was studied. Survival of 100% and 50% were observed respectively on 1st and 5th day post vaccination challenge. The humoral immune genes prophenoloxidase (proPO), alpha 2-macroglobulin (α2M), crustin and PmRACK, and the cell mediated immune genes caspase and Rab7 were up regulated in gill tissue upon vaccination and challenge. The expression of humoral gene crustin and cellular gene Rab7 was related to survival in IVP administered shrimp. Results of the study suggest that these genes have roles in protecting shrimp from WSSV on vaccination.