کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5838216 | 1123977 | 2012 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Saponin rich fractions from Polygonatum odoratum (Mill.) Druce with more potential hypoglycemic effects
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کلمات کلیدی
Saponinα-GlycosidasepNPGMDAPBGGFROGTTSTZRPOCATFBSFBGAUC - AUCDMSO - DMSOMTT - MTTp-nitrophenyl-α-d-glucopyranoside - p-nitrofenyl-a-d-glucopyranosideROS - ROSOral glucose tolerance test - آزمون تحمل گلوکز خوراکیstreptozotocin - استرپتوزوتوسینDiabetes mellitus - دیابت قندیDimethyl sulfoxide - دیمتیل سولفواکسیدSOD - سدfetal bovine serum - سرم جنین گاوSuperoxide dismutase - سوکسوکس دیسموتازFlavonoids - فلاونوئیدهاfasting blood glucose - قند خون ناشتاmalondialdehyde - مالون دی آلدهیدAreas under the curve - مناطق تحت منحنیGlomerular filtration rate - نرخ فیلتراسیون گلومرولیHypoglycemic - هیپوگلیسمیCatalase - کاتالازPostprandial blood glucose - گلوکز خون پس از فروپاشیReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
AimsThe root of Polygonatum odoratum (YuZhu), also a medicinal food has long been used for the treatment of diabetes. The objective of the study was to characterize the anti-diabetic active fractions or compounds in this herb.Materials and methodsFractions with a different polarity were prepared by solvent extraction and macroporous absorptive resin (D101) column and their anti-diabetic potentials were evaluated by glucose uptake in HepG2 cells and STZ-induced diabetic rats. In addition, α-glycosidase inhibitory activities of active fractions were measured in vitro and chemical compositions including saponin, total flavonoids and total sugar in the fractions were determined.ResultsThe n-buthanol fraction, a saponin-rich fraction obtained by partitioning the ethanol extract with n-buthanol after petroleum ether and acetic ether showed the highest anti-diabetic potential in glucose uptake in HepG2 cells followed by acetic ether fraction which was rich in flavonoids. Further fractionation the saponin-rich fraction using macroporous resin column (D101), polysaccharide, flavonoid and saponin rich fractions were obtained by elution with water, 40% and 60% ethanol, respectively and their anti-diabetic potentials proved by glucose uptake test in HepG2 cells and STZ-induced diabetic rats were in the order of saponin rich fraction > flavonoid rich fraction > polysaccharide rich fraction. Long-term therapy test (60 d) in severe diabetic rats indicated that saponin-rich fraction significantly ameliorated clinical symptoms of diabetes including the elevated blood glucose, body weight loss as well as the increased food and water intake while flavonoid-rich fraction was more potential than saponin-rich fraction to increase superoxide dismutase (SOD) activity and decrease malondialdehyde (MDA) level in rat plasma. Additionally, saponin-rich fraction and flavonoid-rich fraction showed α-glycosidase inhibitory activity with IC50 value of 2.05 ± 0.32 and 3.92 ± 0.65 mg/ml, respectively.ConclusionThe results suggested that saponin in this herb was more important than flavonoid in exhibiting anti-diabetic activity and flavonoid contributed more to anti-oxidant activity in vivo.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Ethnopharmacology - Volume 141, Issue 1, 7 May 2012, Pages 228-233
Journal: Journal of Ethnopharmacology - Volume 141, Issue 1, 7 May 2012, Pages 228-233
نویسندگان
Yafei Deng, Kai He, Xiaoli Ye, Xin Chen, Jing Huang, Xuegang Li, Lujiang Yuan, Yalan Jin, Qing Jin, Panpan Li,