کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5841390 1560577 2016 35 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Kaempferol targets Krt-14 and induces cytoskeletal mineralization in osteoblasts: A mechanistic approach
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
Kaempferol targets Krt-14 and induces cytoskeletal mineralization in osteoblasts: A mechanistic approach
چکیده انگلیسی
Kaempferol (KEM) has been observed to stimulate Krt-14 protein which subsequently contributes to matrix maturation and mineralization in rat primary osteoblast cells. Incorporation of Krt-14 siRNA results in reduced mRNA and protein expression after 48 h post transfection and remained low for 9 days. At day 9 Krt-14 siRNA significantly reduced mineralization without concomitant change in the cell number. ColI and OCN gene expression was reduced in Krt-14 siRNA-treated osteoblast cells. Soluble osteocalcin and collagen levels were markedly decreased in conditioned medium as well as in acid-salt soluble cell-ECM layer treated with Krt-14 siRNA compared to control siRNA treated cells corroborated at the ultrastructral level by AFM. Further, knockdown of Krt-14 and inhibitors against AMPK and mTOR, repressed the activation of mTOR and mineralization attenuated by KEM confirmed the role of Krt-14 in mineralization. These findings strongly suggest that Krt-14 regulates osteoblast mineralization by organizing osteoblast derived ECM.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Life Sciences - Volume 151, 15 April 2016, Pages 207-217
نویسندگان
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