کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5856406 1562127 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization and safety evaluation of a Deinococcus member as feed additive for hens
ترجمه فارسی عنوان
ارزیابی و ارزیابی ایمنی یک عضو دینوکوک به عنوان افزودنی خوراکی برای مرغ
کلمات کلیدی
بیوتکنولوژی، سمیت مسمومیت، تخمیر ایمنی مواد غذایی، مواد غذایی رژیمی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی


- GKB-Aid 1995 isolate most resembles Deinococcus grandis using Vitek 2 system and 16S rRNA gene sequencing.
- A Deinococcus member as an ingredient in feed supplements is discussed.
- No adverse or test article-related differences were observed from GKB-Aid 1995 use.

As previous studies mainly focus on understanding the mechanisms of radioresistance in Deinococcus bacteria, the present study aimed at characterizing and verifying the safety use of the GKB-Aid 1995 strain, a member of the radiation-resistant bacterial genus Deinococcus, as an ingredient in feed supplements. Using Vitek 2 system and 16S rRNA gene sequencing, GKB-Aid 1995 most resembles Deinococcus grandis. The Ames test, in vitro chromosomal test, in vivo micronucleus test and acute toxicity test were performed subsequently for its safety evaluation. As there is a possibility that the pigment of GKB-Aid 1995 can pass from feed to eggs intended for human consumption, an acute toxicity test was also carried out in pigmented egg yolk. The results confirmed that GKB-Aid 1995 was non-genotoxic in three genotoxicity experiments, and the LD50 of GKB-Aid 1995 and the pigmented egg yolk in ICR mice was greater than 10 and 12 g kg−1 body weight, respectively. Overall, these data indicate that GKB-Aid 1995 is a non-toxic substance with no genotoxicity and is therefore safe to be used as a feed supplement or feed additive. This study suggests there is potential in developing GKB-Aid 1995 as an animal feed additive intended to enhance yolk coloration to meet the demand of consumers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Regulatory Toxicology and Pharmacology - Volume 76, April 2016, Pages 121-127
نویسندگان
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