کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5858959 | 1562318 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Subacute nicotine co-exposure has no effect on 2,2â²,3,5â²,6- pentachlorobiphenyl disposition but alters hepatic cytochrome P450 expression in the male rat
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CYP2B1qPCRCYP3A2CYP1A2PCBsb.w.P450cytochrome P450 enzymes - آنزیم های سیتوکروم P450Polychlorinated biphenyls - بیفنیل پلیکلر DAY - روزDiatomaceous earth - زمین دیابتیCytochrome P450 - سیتوکروم پی۴۵۰Wistar rat - موش ویستارNicotine - نیکوتین body weight - وزن بدنDisposition - وضعEnantiomeric fraction - کسری انی آنیمیمر
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Polychlorinated biphenyls (PCBs) are metabolized by cytochrome P450 2B enzymes (CYP2B) and nicotine is reported to alter CYP2B activity in the brain and liver. To test the hypothesis that nicotine influences PCB disposition, 2,2â²,3,5â²,6-pentachlorobiphenyl (PCB 95) and its metabolites were quantified in tissues of adult male Wistar rats exposed to PCB 95 (6Â mg/kg/d, p.o.) in the absence or presence of nicotine (1.0Â mg/kg/d of the tartrate salt, s.c.) for 7 consecutive days. PCB 95 was enantioselectively metabolized to hydroxylated (OH-) PCB metabolites, resulting in a pronounced enrichment of E1-PCB 95 in all tissues investigated. OH-PCBs were detected in blood and liver tissue, but were below the detection limit in adipose, brain and muscle tissues. Co-exposure to nicotine did not change PCB 95 disposition. CYP2B1 mRNA and CYP2B protein were not detected in brain tissues but were detected in liver. Co-exposure to nicotine and PCB 95 increased hepatic CYP2B1 mRNA but did not change CYP2B protein levels relative to vehicle control animals. However, hepatic CYP2B protein in animals co-exposed to PCB 95 and nicotine were reduced compared to animals that received only nicotine. Quantification of CYP2B3, CYP3A2 and CYP1A2 mRNA identified significant effects of nicotine and PCB 95 co-exposure on hepatic CYP3A2 and hippocampal CYP1A2 transcripts. Our findings suggest that nicotine co-exposure does not significantly influence PCB 95 disposition in the rat. However, these studies suggest a novel influence of PCB 95 and nicotine co-exposure on hepatic cytochrome P450 (P450) expression that may warrant further attention due to the increasing use of e-cigarettes and related products.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 338, 2 December 2015, Pages 59-68
Journal: Toxicology - Volume 338, 2 December 2015, Pages 59-68
نویسندگان
Marianna Stamou, Eric Uwimana, Brenna M. Flannery, Izabela Kania-Korwel, Hans-Joachim Lehmler, Pamela J. Lein,