کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5859637 | 1562365 | 2012 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparative in vitro study of the inhibition of human and hen esterases by methamidophos enantiomers
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کلمات کلیدی
ToCPIC50Tri-ortho-cresyl phosphateBChENTEOPIDNATCCLD50OECDbutyrylcholinesteraseChiral pesticides - آفت کش ها ChiralOrganophosphate pesticides - آفت کش های ارگانوفسفاتAChE - آهیAcetylcholinesterase - استیل کولین استرازstandard deviation - انحراف معیارCNS - دستگاه عصبی مرکزیOrganisation for Economic Co-operation and Development - سازمان همکاری اقتصادی و توسعهOrganophosphorus pesticide - سموم دفع آفات فسفرcentral nervous system - سیستم عصبی مرکزیMethamidophos - متامیدوفوسAmerican Type Culture Collection - مجموعه فرهنگی نوع آمریکاییmedian lethal dose - میانگین دوز مرگبارneuropathy target esterase - نوراستیک هدف استرازOrganophosphorus-induced delayed neuropathy - نوروپاتی تاخیر ناشی از اتانول فسفرCalpain - کالپین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The current Organisation for Economic Co-operation and Development (OECD) guidelines for evaluating organophosphorus-induced delayed neuropathy (OPIDN) require the observation of dosed animals over several days and the sacrifice of 48 hens. Adhering to these protocols in tests with enantiomers is difficult because large quantities of the compound are needed and many animals must be utilized. Thus, developing an in vitro screening protocol to evaluate chiral organophosphorus pesticides (OPs) that can induce delayed neuropathy is important. This work aimed to evaluate, in blood and brain samples from hens, human blood, and human cell culture samples, the potential of the enantiomeric forms of methamidophos to induce acetylcholinesterase (AChE) inhibition and/or delayed neurotoxicity. Calpain activation was also evaluated in the hen brain and SH-SY5Y human neuroblastoma cells. The ratio between the inhibition of neuropathy target esterase (NTE) and AChE activities by the methamidophos enantiomers was evaluated as a possible indicator of the enantiomers' abilities to induce OPIDN. The (â)-methamidophos exhibited an IC50 value approximately 6 times greater than that of the (+)-methamidophos for the lymphocyte NTE (LNTE) of hens, and (+)-methamidophos exhibited an IC50 value approximately 7 times larger than that of the (â)-methamidophos for the hen brain AChE. The IC50 values were 7 times higher for the human erythrocyte AChE and 5 times higher for AChE in the SH-SY5Y human neuroblastoma cells. Considering the esterases inhibition and calpain results, (+)-methamidophos would be expected to have a greater ability to induce OPIDN than the (â)-methamidophos in humans and in hens.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 292, Issues 2â3, 26 February 2012, Pages 145-150
Journal: Toxicology - Volume 292, Issues 2â3, 26 February 2012, Pages 145-150
نویسندگان
Guilherme L. Emerick, Georgino H. DeOliveira, Regina V. Oliveira, Marion Ehrich,