کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5860319 | 1133178 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces expression of p27kip1 and FoxO3a in female rat cerebral cortex and PC12 cells
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کلمات کلیدی
CDKP27kip1PBSAHRFBSFOXO3aNeuNGFAPTCDDGAPDHRT-PCR2,3,7,8-Tetrachlorodibenzo-p-dioxin - 2،3،7،8-تترا کلریدیبنزوپتوفان دیوکسینSmall interfering RNA - RNA تداخل کوچکROS - ROSsiRNA - siRNANeuronal apoptosis - آپوپتوز عصبیTUNEL - تونلCNS - دستگاه عصبی مرکزیfetal bovine serum - سرم جنین گاوPC12 cells - سلول های PC12central nervous system - سیستم عصبی مرکزیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریRat - موش صحراییneuronal nuclei - هسته های نورونیreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالcyclin dependent kinase - کییناز وابسته به کینازglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازReactive oxygen species - گونههای فعال اکسیژنaryl hydrocarbon receptor - گیرنده آرویل هیدروکربن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces expression of p27kip1 and FoxO3a in female rat cerebral cortex and PC12 cells 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces expression of p27kip1 and FoxO3a in female rat cerebral cortex and PC12 cells](/preview/png/5860319.png)
چکیده انگلیسی
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a potent toxin that alters normal brain development, producing cognitive disability and motor dysfunction. Previous studies in rats have proved that female rats are more sensitive to TCDD lethality than male ones. Recent studies have shown that TCDD induces cell cycle arrest and apoptosis, but the regulatory proteins involved in these processes have yet to be elucidated. In this study, we constructed an acute TCDD injury female rat model, and investigated the effects of TCDD on apoptosis and expression of cell cycle regulators, forkhead box class O 3a (FoxO3a) and p27kip1, in the central nervous system (CNS). Increased levels of active caspase-3 were observed in the cerebral cortex of female rats treated with TCDD, suggesting that TCDD-induced apoptosis occurs in the CNS. The terminal deoxynucleotidyl transferase-mediated biotinylated-dUTP nick-end labeling assay showed that apoptosis primarily occurred in neurons. Furthermore, Western blot analysis, reverse transcription-polymerase chain reaction, and immunohistochemistry showed a significant up-regulation of FoxO3a and p27kip1 in the cerebral cortex. Immunofluorescent labeling indicated that FoxO3a and p27kip1 were predominantly localized in apoptotic neurons, but not in astrocytes. In vitro experiments using PC12, a rat neuron-like pheochromocytoma cell line, also revealed that TCDD induced apoptosis and an increase in FoxO3a and p27kip1 expression. Furthermore, knockdown of FoxO3a expression inhibited p27kip1 transcription and TCDD-induced apoptosis. Based on our data, induction of FoxO3a may play an important role in TCDD-induced neurotoxicity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 226, Issue 3, 2 May 2014, Pages 294-302
Journal: Toxicology Letters - Volume 226, Issue 3, 2 May 2014, Pages 294-302
نویسندگان
Guangfei Xu, Jiao Liu, Katsuhiko Yoshimoto, Gang Chen, Takeo Iwata, Noriko Mizusawa, Zhiqing Duan, Chunhua Wan, Junkang Jiang,