کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5860806 1133241 2011 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gene expression changes in human prostate carcinoma cells exposed to genotoxic and nongenotoxic aryl hydrocarbon receptor ligands
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Gene expression changes in human prostate carcinoma cells exposed to genotoxic and nongenotoxic aryl hydrocarbon receptor ligands
چکیده انگلیسی

Carcinogenic polycyclic aromatic hydrocarbons (PAHs) are known as efficient mutagens and ligands of the aryl hydrocarbon receptor (AhR), which has been suggested to play an important role in prostate carcinogenesis. In order to evaluate the complex relationship between the genotoxicity and the AhR-mediated activity of PAHs in prostate cells, we selected benzo[a]pyrene (BaP) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), as model genotoxic and nongenotoxic AhR ligands, respectively, to explore global changes in gene expression in LNCaP cells by microarray analysis. We identified 112 genes that were differentially expressed in cells treated for 24 h with BaP, TCDD or both compounds. Our data indicated that the impacts of BaP and TCDD on transcriptome of LNCaP cells significantly overlap, since over 64% of significantly up-regulated genes and 47% of down-regulated genes were similarly affected by both AhR ligands. This suggested that the activation of AhR played a prominent role in the nongenotoxic effects of BaP in the prostate carcinoma cell model LNCaP. Both AhR ligands suppressed expression of genes associated with cell cycle progression, DNA replication, spindle assembly checkpoint or DNA repair, which probably occurred secondary to inhibition of cell cycle progression. In contrast, we identified Wnt5a, an important regulator of prostate cancer progression, to be induced as early as 6 h after exposure to both AhR ligands. The AhR ligand-induced Wnt5a upregulation, together with other observed alterations of gene expression, may further contribute to enhanced cell plasticity of prostate carcinoma cells.

► Impacts of BaP (genotoxic AhR ligand) and TCDD (nongenotoxic AhR ligand) on transcriptome of human prostate carcinoma LNCaP cells largely overlap. ► Both types of AhR ligand have a significant impact on genes associated with cell cycle progression, DNA replication, spindle assembly checkpoint, DNA repair or apoptosis regulation, which is primarily related to inhibition of cell proliferation. ► Wnt5a, an important regulator of prostate development and prostate cancer progression, was identified to be induced at mRNA/protein level by both BaP and TCDD in prostate carcinoma cells. ► The activation of AhR plays a prominent role in the nongenotoxic effects of BaP in prostate carcinoma cell model.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 206, Issue 2, 10 October 2011, Pages 178-188
نویسندگان
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