کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5861693 | 1133764 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cigarette-smoke-induced priming of neutrophils from smokers and non-smokers for increased oxidative burst response is mediated by TNF-α
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کلمات کلیدی
MM6MMPPBSLPSN-formyl-methionyl-leucyl-phenylalanineMFIfMLPPMNGM-CSFFCSinterleukin - اینترلوکینPack-years - بسته سالCOPD - بیماری مزمن انسدادی ریهChronic obstructive pulmonary disease - بیماری مزمن انسدادی ریهanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceCigarette smoke - دود سیگارfetal calf serum - سرم گوساله جنینPolymorphonuclear cells - سلولهای پلیمورفونیکmedian fluorescence intensity - شدت فلورسانس متوسطPlatelet activating factor - عامل فعال کننده پلاکتgranulocyte-macrophage colony-stimulating factor - عامل گرانولوسیت-ماکروفاژ colony-stimulating factorlipopolysaccharide - لیپوپلی ساکاریدmatrix metalloproteinase - ماتریکس متالوپروتئینازPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریPAF - نیروی هوایی پاکستانPropidium iodide - پروتئین یدید
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In vitro treatment of human peripheral blood neutrophils from smokers and non-smokers with an aqueous cigarette smoke (CS) extract resulted in a concentration-dependent increase in surface expression of CD11b and CD66b and a corresponding decrease of CD62L, together with a concentration-dependent release of MMP-8, MMP-9, and lactoferrin, indicating considerable activation and degranulation. However, the burst response to N-formyl-methionyl-leucyl-phenylalanine (fMLP) was unchanged in CS-stimulated neutrophils from both smokers and non-smokers. When supernatants from CS-treated monocytic MonoMac-6 (MM6) cells were used for activation of neutrophils, concentration-dependent changes in surface marker expression, granule protein release, and the oxidative burst response to fMLP were observed, again with no major differences between smokers and non-smokers. CS-treated MM6 cells released significant amounts of IL-8 and TNF-α into the culture supernatant. However, antibody blocking experiments showed that only TNF-α mediated the increased burst response in neutrophils. These data show that, in the presence of secondary cells, CS is able to prime neutrophils for an increased burst response to fMLP which is mediated by TNF-α, released from the secondary cells in response to CS. Following stimulation with priming agents, peripheral blood neutrophils from healthy smokers show an equal burst response compared to those from non-smokers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 28, Issue 7, October 2014, Pages 1249-1258
Journal: Toxicology in Vitro - Volume 28, Issue 7, October 2014, Pages 1249-1258
نویسندگان
Bärbel Friedrichs, Ute Neumann, Jutta Schüller, Michael J. Peck,