کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5861778 | 1133765 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro microarray analysis identifies genes in acute-phase response pathways that are down-regulated in the liver of chicken embryos exposed in ovo to PFUdA
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کلمات کلیدی
PFCFGGcarboxypeptidase B2CPB2PROCHNF4ACEHA2MGSTO1PFNATP53PFCAPLGPFDSFGAIPAMGST1PFOAPFOSperfluorodecane sulfonategsta4FDRGSHPPARγPPARαDMSO - DMSOalpha-2-macroglobulin - آلفا 2-ماگروگلوبولینApr - آوریلPerfluorocarboxylic acid - اسید PerfluorocarboxylicPerfluorononanoic acid - اسید PerfluorononanoicPerfluoroundecanoic acid - اسید PerfluoroundecanoicGene-expression - بیان ژنIngenuity Pathway Analysis - تجزیه و تحلیل راه IngenuityMode of action - حالت عملDimethyl sulfoxide - دیمتیل سولفواکسیدPerfluorooctane sulfonate - سولفونات Perfluorooctanehepatocyte nuclear factor 4 alpha - عامل هسته ای هپاتوسیت 4 آلفاDifferentially expressed - متفاوت بیان شده استChicken - مُرغ خانگیfalse discovery rate - میزان کشف کاذبHepatocyte - هپاتوسیتAcute-phase response - پاسخ فاز حادPerfluorooctanoic acid - پرفلوئورواکتانوئیک اسیدProtein C - پروتئین Ctumor protein 53 - پروتئین تومور 53Plasminogen - پلاسمینوژنPeroxisome proliferator-activated receptor gamma - گاما گیرنده گیرنده فعال پرولیفیزوم فعالGlutathione - گلوتاتیون
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Perfluoroundecanoic acid (PFUdA) is one of the most highly detected perfluoroalkyl compounds in wild bird tissues and eggs. Although PFUdA does not affect hatching success, many PFCs are known to impair post-hatch development and survival. Here we use microarrays to survey the transcriptional response of cultured chicken embryonic hepatocytes (CEH) to PFUdA for potential targets of PFUdA action that could lead to developmental deficiencies in exposed birds. At 1 μM and 10 μM PFUdA significantly altered the expression of 346 and 676 transcripts, respectively (fold-change > 1.5, p < 0.05, false discovery rate-corrected). Using functional, pathway and interactome analysis we identified several potentially important targets of PFUdA exposure, including the suppression of the acute-phase response (APR). We then measured the expression of five APR genes, fibrinogen alpha (fga), fibrinogen gamma (fgg), thrombin (f2), plasminogen (plg), and protein C (proC), in the liver of chicken embryos exposed in ovo to PFUdA. The expression of fga, f2, and proC were down-regulated in embryo livers (100 or 1000 ng/g, p < 0.1) as predicted from microarray analysis, whereas fibrinogen gamma (fgg) was up-regulated and plg was not significantly affected. Our results demonstrate the utility of CEH coupled with transcriptome analysis as an in vitro screening tool for identifying novel effects of toxicant exposure. Additionally, we identified APR suppression as a potentially important and environmentally relevant target of PFUdA. These findings suggest in ovo exposure of birds to PFUdA may lead to post-hatch developmental deficiencies, such as impaired inflammatory response.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 27, Issue 6, September 2013, Pages 1649-1658
Journal: Toxicology in Vitro - Volume 27, Issue 6, September 2013, Pages 1649-1658
نویسندگان
Jason M. O'Brien, Andrew Williams, Carole L. Yauk, Doug Crump, Sean W. Kennedy,