An in vitro tool to assess cytochrome P450 drug biotransformation-dependent cytotoxicity in engineered HepG2 cells generated by using adenoviral vectors

► We have developed an in vitro tool to detect bioactivation-dependent toxicity. ► We can identify CYP enzymes involved in metabolism-based toxicity. ► We are able to modulate expression of a given CYP using adenovirus. ► Our model allows the rapid analysis for curve fitting and IC50 generation in early toxicity.