کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5862299 | 1133775 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Screening of dioxin-like compounds by complementary evaluation strategy utilising ELISA, micro-EROD, and HRGC-HRMS in soil and sediments from Montevideo, Uruguay
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کلمات کلیدی
PBSPCDFPCDDTCDDERODPBSTTMBASEDulbecco’s Minimum Essential MediumPCBPAHTMDDDMEM2,3,7,8-Tetrachlorodibenzo-p-dioxin - 2،3،7،8-تترا کلریدیبنزوپتوفان دیوکسین3,3′,5,5′-tetramethylbenzidine - 3،3 '، 5،5'-تترامیلیل بنزیدینBSA - BSAPolychlorinated dibenzofurans - dibenzofurans پلی کربناتHRGC/HRMS - HRGC / HRMSbovine serum albumin - آلبومین سرم گاوPolychlorinated biphenyls - بیفنیل پلیکلر Dioxin-like compounds - ترکیبات دیوکسین مانندELISA - تست الیزاSoil - خاکPolychlorinated dibenzo-p-dioxins - دی بنزوپروتئینهای پلی کربناتSediment - رسوبaccelerated solvent extraction - شتاب دهنده استخراج حلالRelative potency - قدرت نسبیphosphate buffer solution - محلول بافر فسفاتCut-off values - مقادیر برشRep - هرزهPolycyclic aromatic hydrocarbons - هیدروکربن آروماتیک چندحلقهایCross-reactivity - واکنش پذیری متقابل
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Screening of dioxin-like compounds by complementary evaluation strategy utilising ELISA, micro-EROD, and HRGC-HRMS in soil and sediments from Montevideo, Uruguay Screening of dioxin-like compounds by complementary evaluation strategy utilising ELISA, micro-EROD, and HRGC-HRMS in soil and sediments from Montevideo, Uruguay](/preview/png/5862299.png)
چکیده انگلیسی
Polychlorinated dibenzo-p-dioxins (PCDD) and dibenzofurans (PCDF) are persistent, toxic, and bioaccumulate in the environment. Due to their high analytical costs, these compounds are hardly regulated and mostly not monitored in the Third World. To overcome this, bioassays have been proposed as low-cost alternative methods. Two of the most established bioanalytical tools, the dioxin antibody-based enzyme-linked immunosorbent assay ELISA and the micro-EROD bioassay are evaluated and compared to high resolution gas chromatography and high resolution mass spectrometry (HRGC/HRMS) analytical methodology. The methods were tested using thirteen soils and sediment samples selected from diverse sites in Montevideo, Uruguay. The WHO2005 total toxic equivalent (WHO2005-TEQ) of soils ranged from 2.4 to 2212 (ng WHO2005-TEQ/kg dry sample) and from 0.14 to 9.4 (ng WHO2005-TEQ/kg dry sample) in sediments. This study shows significant contamination related to dioxin-like compounds, particularly in sites where uncontrolled burnings were carried out. ELISA and micro-EROD bioassay correlated well with HRGC/HRMS, R Spearman 0.773 and 0.913, respectively and were highly correlated to each other, R Spearman 0.879. Preliminary threshold values of bioassay toxic equivalents of 330 (ng/kg dry sample) for the micro-EROD bioassay and 220 (ng/kg dry sample) for ELISA are proposed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 28, Issue 5, August 2014, Pages 1036-1045
Journal: Toxicology in Vitro - Volume 28, Issue 5, August 2014, Pages 1036-1045
نویسندگان
W. Levy, B.M. Brena, B. Henkelmann, S. Bernhöft, M. Pirez, G. González-Sapienza, K.-W. Schramm,