کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5907611 | 1569857 | 2011 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MicroRNA-146a is linked to pain-related pathophysiology of osteoarthritis
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کلمات کلیدی
MMPTLRiNOSBCAtransient receptor potential cation channel, subfamily V, member 1COX-2MIAIRAK1DRGADAMTSIL-1NFkBTRPV1LPSUTRsTNFRheumatoid arthritis - آرتریتروماتوئیدOsteoarthritis - استئوآرتریت(آرتروز)interleukin-1 - اینترلوکین-1miR-146a - به miR-146Abicinchoninic acid - بیسینکنینیک اسیدa disintegrin and metalloproteinase with thrombospondin motifs - تخریب و متالوپروتئیناز با ترومبوسپوندین موتیفToll-like receptor - تیالآرPain - دردreverse transcription - رونویسی معکوسglial cells - سلول های گلیالSynovial cells - سلولهای سینوویوCyclooxygenase-2 - سیکلوکوکسیژناز2TNF receptor-associated factor 6 - عامل گیرنده TNF 6tumor necrosis factor - فاکتور نکروز تومورNuclear factor-kappa B - فاکتور هسته ای-کاپا Blipopolysaccharide - لیپوپلی ساکاریدmatrix metalloproteinase - ماتریکس متالوپروتئینازUntranslated regions - مناطق غیر ترجمهpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازChondrocyte - کندروسیتdorsal root ganglia - گانگلیس ریشه پشتیmonosodium iodoacetate - یون ازدیاد مونو سدیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Because miR-146a is linked to osteoarthritis (OA) and cartilage degeneration is associated with pain, we have characterized the functional role of miR-146a in the regulation of human articular cartilage homeostasis and pain-related factors. Expression of miRNA 146a was analyzed in human articular cartilage and synovium, as well as in dorsal root ganglia (DRG) and spinal cord from a rat model for OA-related pain assessment. The functional effects of miR-146a on human chondrocytic, synovialm and microglia cells were studied in cells transfected with miR-146a. Using real-time PCR, we assessed the expression of chondrocyte metabolism-related genes in chondrocytes, genes for inflammatory factors in synovial cells, as well as pain-related proteins and ion channels in microglial cells. Previous studies showed that miR-146a is significantly upregulated in human peripheral knee OA joint tissues. Transfection of synthetic miR-146a significantly suppresses extracellular matrix-associated proteins (e.g., Aggrecan, MMP-13, ADAMTS-5, collagen II) in human knee joint chondrocytes and regulates inflammatory cytokines in synovial cells from human knee joints. In contrast, miR-146a is expressed at reduced levels in DRGs and dorsal horn of the spinal cords isolated from rats experiencing OA-induced pain. Exogenous supplementation of synthetic miR-146a significantly modulates inflammatory cytokines and pain-related molecules (e.g., TNFα, COX-2, iNOS, IL-6, IL8, RANTS and ion channel, TRPV1) in human glial cells. Our findings suggest that miR-146a controls knee joint homeostasis and OA-associated algesia by balancing inflammatory responses in cartilage and synovium with pain-related factors in glial cells. Hence, miR-146a may be useful for the treatment of both cartilage regeneration and pain symptoms caused by OA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 480, Issues 1â2, 1 July 2011, Pages 34-41
Journal: Gene - Volume 480, Issues 1â2, 1 July 2011, Pages 34-41
نویسندگان
Xin Li, Gary Gibson, Jae-Sung Kim, Jeffrey Kroin, Shunbin Xu, Andre J. van Wijnen, Hee-Jeong Im,