کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5907856 | 1570101 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Direct ChIP-bisulfite sequencing reveals a role of H3K27me3 mediating aberrant hypermethylation of promoter CpG islands in cancer cells
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کلمات کلیدی
STADPRCCGICOADH3K27me3PRADRPKMhistone H3 lysine 27TSSs - TSS هاstomach adenocarcinoma - آدنوکارسینوم معدهProstate adenocarcinoma - آدنوکارسینوم پروستاتColon adenocarcinoma - آدنوکارسینوم کولونchromatin immunoprecipitation - ایمن سازی کروماتینreads per kb per million reads - به ازای هر میلیون نسخه خوانده می شودCpG island - جزیره CpGtranscription start sites - رونویسی سایت های شروعGastric cancer - سرطان معدهDNA methylation - متیلاسیون DNA
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The model describing that aberrant CpG island (CGI) methylation leads to repression of tumour suppressor genes in cancers has been influential, but it remains unclear how such aberrancy is induced. Recent studies provided clues indicating that promoter hypermethylation in cancers might be associated with PRC target genes. Here, we used ChIP-BS-seq to examine methylation of the DNA fragments precipitated by the antibodies to both H3K27me3 and H3K4me3 histone modifications. We showed that, for a set of genes highly enriched with H3K27me3 both in cancer and normal cells, CGI promoters were aberrantly hypermethylated only in cancer cells in comparison with normal cells. In contrast, such aberrant CGI hypermethylation in cancer promoters that were deficient of H3K27me3 was not notable. Furthermore, we confirmed that these genes were consistently hypermethylated in TCGA primary cancer cells. These works support the association between H3K27me3 and DNA methylation marks for specific cancer genes and will spur future work on combined histone and DNA methylation that could define cancer's epigenetic abnormalities.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics - Volume 103, Issues 2â3, FebruaryâMarch 2014, Pages 204-210
Journal: Genomics - Volume 103, Issues 2â3, FebruaryâMarch 2014, Pages 204-210
نویسندگان
Fei Gao, Guanyu Ji, Zhaowei Gao, Xu Han, Mingzhi Ye, Zhimei Yuan, Huijuan Luo, Xiaojun Huang, Karthikraj Natarajan, Jun Wang, Huanming Yang, Xiuqing Zhang,