Polyethylenimine attached-poly(3-chloro-2-hydroxypropyl methacrylate-co-ethylene dimethacrylate) monosized-porous microspheres as a new separation medium for polar compounds

- Chloropropyl functionalized reactive, hydrophilic polymer beads were newly synthesized.
- A macromolecular ligand, polyethylenimine was attached onto the hydrophilic beads.
- Polyethylenimine attached beads were used as stationary medium in HILIC mode.
- Polar compounds and biomolecules compounds were successfully separated in micro-LC.
- Theoretical plate numbers up to 23,000 plates/m were achieved.