کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6021263 | 1580626 | 2016 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulation of therapeutic hypothermia on inflammatory cytokines, microglia polarization, migration and functional recovery after ischemic stroke in mice
ترجمه فارسی عنوان
مقررات هیپوترمی درمانی بر سیتوکین های التهابی، قطبی شدن میکروگلای، مهاجرت و بهبود عملکرد پس از سکته مغزی ایسکمیک در موش
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
NTR1IFNγPIHIBA1IOSIP-10IL-12IL-10IL-1βMCP-1MIP-1αiNOSTTCCCAIL-23TTMIL-6M1/M2 polarization2,3,5-triphenyltetrazolium chloride - 2،3،5-trihenyltetrazolium chlorideinflammation - التهاب( توروم) interleukin-12 - اینترلوکین -12Interleukin-23 - اینترلوکین -23Interleukin-10 - اینترلوکین 10interleukin-6 - اینترلوکین ۶Interleukin-1β - اینترلوکین-1βFunctional recovery - بهبود عملکردanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of variancetumor necrosis factor-α - تومور نکروز عامل αBBB - سد خونی مغزیinducible nitric oxide synthase - سنتاز اکسید نیتریک القاییStroke - سکته مغزیIntrinsic optical signals - سیگنال های نوری ذاتیcommon carotid artery - شریان کاروتید مشترکTNF-α - فاکتور نکروز توموری آلفاBlood-brain barrier - مانع خون مغزیTargeted temperature management - مدیریت درجه حرارت هدفionized calcium binding adaptor molecule 1 - مولکول آداپتور اتصال دهنده کلسیم یونیزه 1neurotensin - نوروتنسینHypothermia - هیپوترمیTherapeutic hypothermia - هیپوترمی درمانیMacrophage inflammatory protein-1α - پروتئین التهابی ماکروفاژ 1αinterferon-inducible protein - پروتئین القا کننده اینترفرونmonocyte chemoattractant protein-1 - پروتئین شیمیایی monocyte chemoattractant-1Interferon gamma - گاما اینترفرونneurotensin receptor 1 - گیرنده نورونسین 1
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
عصب شناسی
چکیده انگلیسی
Stroke is a leading threat to human life and health in the US and around the globe, while very few effective treatments are available for stroke patients. Preclinical and clinical studies have shown that therapeutic hypothermia (TH) is a potential treatment for stroke. Using novel neurotensin receptor 1 (NTR1) agonists, we have demonstrated pharmacologically induced hypothermia and protective effects against brain damages after ischemic stroke, hemorrhage stroke, and traumatic brain injury (TBI) in rodent models. To further characterize the mechanism of TH-induced brain protection, we examined the effect of TH (at ± 33 °C for 6 h) induced by the NTR1 agonist HPI-201 or physical (ice/cold air) cooling on inflammatory responses after ischemic stroke in mice and oxygen glucose deprivation (OGD) in cortical neuronal cultures. Seven days after focal cortical ischemia, microglia activation in the penumbra reached a peak level, which was significantly attenuated by TH treatments commenced 30 min after stroke. The TH treatment decreased the expression of M1 type reactive factors including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-12, IL-23, and inducible nitric oxide synthase (iNOS) measured by RT-PCR and Western blot analyses. Meanwhile, TH treatments increased the expression of M2 type reactive factors including IL-10, Fizz1, Ym1, and arginase-1. In the ischemic brain and in cortical neuronal/BV2 microglia cultures subjected to OGD, TH attenuated the expression of monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1α (MIP-1α), two key chemokines in the regulation of microglia activation and infiltration. Consistently, physical cooling during OGD significantly decreased microglia migration 16 h after OGD. Finally, TH improved functional recovery at 1, 3, and 7 days after stroke. This study reveals the first evidence for hypothermia mediated regulation on inflammatory factor expression, microglia polarization, migration and indicates that the anti-inflammatory effect is an important mechanism underlying the brain protective effects of a TH therapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurobiology of Disease - Volume 96, December 2016, Pages 248-260
Journal: Neurobiology of Disease - Volume 96, December 2016, Pages 248-260
نویسندگان
Jin Hwan Lee, Zheng Z Wei, Wenyuan Cao, Soonmi Won, Xiaohuan Gu, Megan Winter, Thomas A. Dix, Ling Wei, Shan Ping Yu,