کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6113932 | 1213511 | 2016 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of in vitro storage characteristics of cold stored platelet concentrates with N acetylcysteine (NAC)
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کلمات کلیدی
Sialidase activityPrPMoAbPFARTCPSLNACGSHPlatelet storage lesionRFUROS - ROSstandard deviation - انحراف معیارRewarming - حرارت دادنWhole blood - خون کاملStorage - ذخیره سازیPCs - رایانه های شخصیphycoerythrin - فایکوئیریدینPhosphatidylserine - فسفاتیدیلسرینNitric oxide - نیتریک اکسیدrelative fluorescence unit - واحد فلورسانس نسبیMonoclonal antibodies - پادتنهای تَکتیرهparaformaldehyde - پارافرمالدهیدPlatelet rich plasma - پلاسما غنی از پلاکتPlatelet concentrates - پلاکت کنسانترهGlutathione - گلوتاتیونReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
هماتولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Platelets play a vital role in hemostasis and thrombosis, and their demand and usage has multiplied many folds over the years. However, due to the short life span and storage constraints on platelets, it is allowed to store them for up to 7 days at room temperature (RT); thus, there is a need for an alternative storage strategy for extension of shelf life. Current investigation involves the addition of 50âmM N acetylcysteine (NAC) in refrigerated concentrates. Investigation results revealed that addition of NAC to refrigerated concentrates prevented platelet activation and reduced the sialidase activity upon rewarming as well as on prolonged storage. Refrigerated concentrates with 50âmM NAC expressed a 23.91â±â6.23% of CD62P (P-Selectin) and 22.33â±â3.42% of phosphotidylserine (PS), whereas RT-stored platelets showed a 46.87â±â5.23% of CD62P and 25.9â±â6.48% of phosphotidylserine (PS) after 5 days of storage. Further, key metabolic parameters such as glucose and lactate accumulation indicated reduced metabolic activity. Taken together, investigation and observations indicate that addition of NAC potentially protects refrigerated concentrates by preventing platelet activation, stabilizing sialidase activity, and further reducing the metabolic activity. Hence, we believe that NAC can be a good candidate for an additive solution to retain platelet characteristics during cold storage and may pave the way for extension of storage shelf life.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Transfusion and Apheresis Science - Volume 54, Issue 1, February 2016, Pages 127-138
Journal: Transfusion and Apheresis Science - Volume 54, Issue 1, February 2016, Pages 127-138
نویسندگان
Mallikarjun Handigund, Tae Won Bae, Jaehyeon Lee, Yong Gon Cho,