کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6117065 | 1217159 | 2015 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Prime/boost immunization with HIV-1 MPER-V3 fusion construct enhances humoral and cellular immune responses
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کلمات کلیدی
APCsENVNLSBNAbsMPERCPPs - CPP هاBroadly neutralizing antibodies - آنتی بادی های ضد عفونی به طور گسترده ایAntigen presenting cells - آنتیژن ارائه سلولnuclear localization sequence - دنباله محلی سازی هسته ایmembrane-proximal external region - غشاء-پروگزیمال خارجی ناحیهDNA vaccine - واکسن DNA Peptide vaccine - واکسن پپتیدHIV - ویروس نقص ایمنی انسانی Envelope - پاکت نامهCell penetrating peptides - پپتیدهای نفوذ سلولیGlycoprotein - گلیکوپروتئین
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Development of an effective vaccine against HIV-1 infection is a main concern in worldwide. A potent vaccine for HIV-1 requires the induction and maintenance of both humoral and cellular immunity. In this study, the levels of humoral and cellular immune responses were compared using MPER-V3 injection in three immunization strategies such as DNA/DNA, peptide/peptide, and DNA/peptide (prime-boost). MPG peptide and Montanide 720 were used as a DNA delivery system, and as a peptide adjuvant, respectively. Our results demonstrated that MPG forms stable non-covalent nanoparticles with plasmid DNA at N/P ratio of 10:1 (â¼110-130 nm). The in vitro transfection efficiency of MPER-V3 DNA using MPG was comparable with lipofectamine and turbofect reagents as a common delivery system. In vivo prime-boost immunization using HIV-1 MPER-V3 could significantly enhance humoral and cellular immune responses as compared to control groups. The mixture of IgG1 and IgG2a was observed for each strategy, but IFN-γ production was significantly higher in prime-boost and peptide immunizations than that in DNA immunizations, inducing Th1 response. Moreover, our data showed that prime immunization with low dose of the nanoparticles (MPER-V3 DNA: MPG at ratio of 1:10) followed by MPER-V3 peptide drives T cell responses towards a Th1-type similar to high dose of the naked DNA prime/peptide boost immunization. Generally, the prime-boost strategy could improve both immune responses against MPER and especially V3 peptides suggesting its application as a promising HIV vaccine candidate in future.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunology Letters - Volume 168, Issue 2, December 2015, Pages 366-373
Journal: Immunology Letters - Volume 168, Issue 2, December 2015, Pages 366-373
نویسندگان
Azam Bolhassani, Kimia Kardani, Rouhollah Vahabpour, Nourieh Habibzadeh, Mohammad Reza Aghasadeghi, Seyed Mehdi Sadat, Elnaz Agi,