کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6117148 | 1217167 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lack of CD24 expression in mice reduces the number of leukocytes in the colon
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کلمات کلیدی
CD24GPimAbFBSEAEHSAMonoclonal antibody - آنتی بادی مونوکلونالheat-stable antigen - آنتی ژن پایدار گرماexperimental autoimmune encephalomyelitis - آنسفالومیلیت خودایمنی تجربیIHC - ایمونوهیستوشیمیImmunohistochemistry - ایمونوهیستوشیمیIntegrins - اینتگرینCNS - دستگاه عصبی مرکزیfetal bovine serum - سرم جنین گاوCytokines - سیتوکین هاcentral nervous system - سیستم عصبی مرکزیDetergent resistant membranes - غشای مقاوم در برابر مواد شویندهwildtype - نوع وحشیChemokines - کرموین هاglycosyl-phosphatidylinositol - گلیکوزیل فسفاتیدیلینوزیتول
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
CD24 is an extensively glycosylated membrane protein that is linked to the membrane via a glycosyl-phosphatidylinositol (GPI)-anchor. In mice, CD24 is expressed by hematopoietic and non-hematopoietic cells. CD24â/â mice do not have gross immunological defects, but detailed analysis revealed strongly reduced responses in an experimental autoimmune encephalomyelitis (EAE) model and a massive proliferation of T cells under lymphopenic conditions. It was also demonstrated that preB cells from CD24â/â mice are impaired in α4-integrin-mediated cell binding. Here we report that CD24â/â mice have strongly reduced numbers of leukocytes in the colon compared to wildtype mice. The reduction comprized all subpopulations. Leukocyte counts in spleen, mesenteric lymph nodes or small intestine were not significantly different. We find that beside leukocytes, CD24 is widely expressed in EpCAM+ epithelial and CD31+ endothelial cells of colon and small intestine. However, in CD24â/â mice the number of CD31+ endothelial cells in colons was strongly reduced and the number of epithelial cells was augmented. Leukocyte transfer experiments provided evidence that the CD24 status of recipient mice, rather than of the transferred cells, is crucial for leukocyte recruitment to the colon. We hypothesize that CD24 on colonic epithelial and endothelial cells is required for the retention and positioning of leukocytes most likely by affecting integrin function.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunology Letters - Volume 161, Issue 1, September 2014, Pages 140-148
Journal: Immunology Letters - Volume 161, Issue 1, September 2014, Pages 140-148
نویسندگان
Niko P. Bretz, Alexei V. Salnikov, Kai Doberstein, Natalio Garbi, Volker Kloess, Safwan Joumaa, Inna Naumov, Louis Boon, Gerhard Moldenhauer, Nadir Arber, Peter Altevogt,