Isolation and characterization of a human antibody fragment specific for Ts1 toxin from Tityus serrulatus scorpion

► A single chain human antibody (scFv) was selected by bio-panning of a phage-display library. ► The clones were selected for their capacity to recognize toxin Ts1 from the scorpion Tityus serrulatus. ► Clone 15e was capable of recognizing and partially protecting mice from the toxic effect of Ts1. ► The single chain antibody (scFv) was VH6-VK4, containing 124 amino acids for VH6 and 114 for VK4. ► The clone 15e also recognizes toxins from other species of dangerous scorpions of the same genus from the Amazonic region. ► It is a leading compound for the development of neutralizing antibodies of human origin against scorpion stings in South American countries.