کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6133143 | 1593456 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of a colloidal gold immunochromatographic strip for the rapid detection of soft-shelled turtle systemic septicemia spherical virus
ترجمه فارسی عنوان
توسعه طیف ایمونوکلروماتوگرافی طلا کلوئیدی برای تشخیص سریع لاک پشتی لاک پشت سیستمیک ویروس کروی سپتی سمی
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کلمات کلیدی
PcAbTNESTSSEgg yolk antibody (IgY)p.i.ICSPMSFBSA - BSAIgY - IgY بهbovine serum albumin - آلبومین سرم گاوEgg yolk antibody - آنتی بادی زرده تخم مرغPolyclonal antibody - آنتی بادی های پلی کلونالTem - این استPost Injection - تزریق پستTest - تستELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاLOD یا Limit of detection - حد تشخیصHour - ساعتphenylmethylsulfonyl fluoride - فنیل متیل سولفونیل فلورایدlimit of detection - محدودیت تشخیصTransmission electron microscopy - میکروسکوپ الکترونی عبوریGold nanoparticle - نانو ذرات طلاImmunochromatographic strip - نوار ایمونو کروماتوگرافیControl - کنترل
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
چکیده انگلیسی
A colloidal gold immunochromatographic strip (ICS) test based on a competitive format was developed for the rapid detection of soft-shelled turtle systemic septicemia spherical virus (STSSSV) in turtle and fecal samples. Specific egg yolk antibodies (IgY) against STSSSV were labeled with colloidal gold and used as probes in the one-step test strip. Antigen (STSSSV) and goat anti-chicken IgY were drawn on the nitrocellulose membrane as the test line and control line, respectively. When STSSSV standard samples (0-100 μg/mL) were detected by the strips, the visual limit of detection (LOD) was found to be 50.0 μg/mL. The ICS test showed high stability; the strips were stable for at least 3 months at 4 °C without significant loss of activity. There was no obvious cross-reactivity with other aquatic pathogens. The assay can be performed within 5-10 min. Analysis of STSSSV in turtle samples revealed that data obtained from the ICS test were in a good agreement with those obtained by ELISA. The positive results of fecal samples suggested that this method could be used to detect STSSSV while protecting the animals' welfare. The ICS assay does not need specialized equipment or a technician and can be used as a reliable, rapid, cost-effective and convenient qualitative tool for on-site diagnosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 221, 1 September 2015, Pages 39-45
Journal: Journal of Virological Methods - Volume 221, 1 September 2015, Pages 39-45
نویسندگان
Liping Zhang, Dengfeng Li, Lianguo Liu, Jing Fang, Ran Xu, Ge Zhang,