کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6133403 | 1593462 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro assessment of the effect of vaccine-targeted human papillomavirus (HPV) depletion on detection of non-vaccine HPV types: Implications for post-vaccine surveillance studies
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
hrHPVDNA - DNA یا اسید دزوکسی ریبونوکلئیکdeoxyribonucleic acid - اسید deoxyribonucleicGenotyping - تعیین ساختار ژنتیکیLOD یا Limit of detection - حد تشخیصlimit of detection - محدودیت تشخیصFalse negatives - منفی های دروغینSurveillance - نظارتVaccine - واکسنHuman papillomavirus - ویروس پاپیلوم انسانیHigh risk human papillomavirus - ویروس پاپیلومای با خطر بالاHPV - ویروس پایپلوم انسانی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: In vitro assessment of the effect of vaccine-targeted human papillomavirus (HPV) depletion on detection of non-vaccine HPV types: Implications for post-vaccine surveillance studies In vitro assessment of the effect of vaccine-targeted human papillomavirus (HPV) depletion on detection of non-vaccine HPV types: Implications for post-vaccine surveillance studies](/preview/png/6133403.png)
چکیده انگلیسی
In populations where the prevalence of vaccine-targeted HPV types has been reduced significantly due to widespread vaccination of the target population, the sensitivity of some consensus PCR-based assays to detect remaining HPV types may be altered, leading to misrepresentations of prevalence. Importantly, this may lead to false indications of type replacement in vaccinated populations. To assess whether excess vaccine-targeted HPV DNA resulted in reduced detection of other genotypes on the Roche HPV linear array genotype assay, simulated samples containing 1000 copies of one or two high-risk HPV DNA genomes in the presence and the absence of 10,000 copies of the HPV16 genome were tested. HPV16 alone did not affect detection of other high-risk genotypes; however when HPV16 and an additional genotype were present, detection of HPV31, 33, 51 or 59 was impeded, indicating potential for misrepresentation of population-based prevalence of these genotypes and false evidence for type replacement following vaccination.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 214, 15 March 2015, Pages 10-14
Journal: Journal of Virological Methods - Volume 214, 15 March 2015, Pages 10-14
نویسندگان
Alyssa M. Cornall, Samuel Phillips, Eleanor Cummins, Suzanne M. Garland, Sepehr N. Tabrizi,