کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6135690 | 1593639 | 2013 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Interaction of macrophages with a cytotoxic Serratia liquefaciens human isolate
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Interaction of macrophages with a cytotoxic Serratia liquefaciens human isolate Interaction of macrophages with a cytotoxic Serratia liquefaciens human isolate](/preview/png/6135690.png)
چکیده انگلیسی
Macrophages play key roles in host defense by recognizing, engulfing, and killing microorganisms. Understanding the response of macrophages to pathogens may provide insights into host defenses and the tactics used by pathogens to circumvent these defenses. In the present study, we investigated the interaction between a clinical isolate of Serratia liquefaciens and macrophages. S. liquefaciens strain HUMV-3250 triggers a fast and potent cytotoxic effect upon infection. This process requires the presence of live bacteria, adherence, and protein synthesis but not phagocytosis/bacterial internalization. Moreover, cytotoxicity assays, analysis of DNA integrity, immunofluorescence, and confocal, scanning, and time-lapse microscopy revealed that macrophage viability decreased rapidly with time upon challenge, and depends on the MOI used. Treatment of macrophages with caspase-1 inhibitors, or with specific inhibitors of phagocytosis, did not alter the infection outcome. Moreover, human macrophages exhibited similar cytotoxic changes after infection with this strain. Macrophages responded to this cytotoxic strain with a robust pattern of pro-inflammatory gene expression. However, phagocytosis attempts to engulf live bacteria were unsuccessful, and the phagocytes were unable to kill the bacteria. We conclude that macrophage cell death occurs rapidly as a result of necrotic events after close contact with S. liquefaciens. These results likely have important implications for understanding Serratia pathogenesis and host response to infection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbes and Infection - Volume 15, Issues 6â7, June 2013, Pages 480-490
Journal: Microbes and Infection - Volume 15, Issues 6â7, June 2013, Pages 480-490
نویسندگان
Sara Remuzgo-MartÃnez, Maitane Aranzamendi-Zaldunbide, Lilian Pilares-Ortega, José Manuel Icardo, Félix Acosta, Luis MartÃnez-MartÃnez, José Ramos-Vivas,