کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6135833 | 1224905 | 2012 | 11 صفحه PDF | دانلود رایگان |
Japanese encephalitis virus (JEV) is a flavivirus with a complex life cycle involving mosquito vectors that mainly target birds and pigs, and causes severe encephalitis in children in Asia. Neurotropic flaviviruses of the JEV serogroup have a particular characteristic of expressing a unique nonstructural NS1â² protein, which is a prolongation of NS1 at the C terminus by 52 amino acids derived from a pseudoknot-driven-1 translation frameshift. Protein NS1â² is associated with virus neuro-invasiveness. In this study, the need of the pseudoknot structure for NS1â² synthesis was confirmed. By using a specific antibody against the prolonged peptide, NS1â² was found to be absent from the JEV SA14-14-2 vaccine strain, resulting from a single nucleotide silent mutation in the pseudoknot. A partial cleavage of NS1â² at a specific site of its C-terminal appendix recognized by caspases and inhibited by caspase inhibitors suggests a unique feature of intracellular NS1â².
Journal: Microbes and Infection - Volume 14, Issue 11, September 2012, Pages 930-940